Quorum sensing between Pseudomonas aeruginosa biofilms accelerates cell growth.
Quorum sensing between Pseudomonas aeruginosa biofilms accelerates cell growth.
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DOI：
10.1021/ja111131f
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发表时间：
2011-04-20
影响因子：
15
通讯作者：
Weibel, Douglas B.
中科院分区：
文献类型：
--
作者：
Flickinger, Shane T.;Copeland, Matthew F.;Downes, Eric M.;Braasch, Andrew T.;Tuson, Hannah H.;Eun, Ye-Jin;Weibel, Douglas B.
这篇手稿描述了制造的阵列的空间限制室浮雕在一层的聚（乙二醇）二丙烯酸酯（PEGDA）和它们的应用研究群体感应之间的铜绿假单胞菌。我们假设，生物膜可能会产生稳定的化学信号梯度在接近表面，影响附近的小菌落的生长和发育成生物膜。为了验证这一假设，我们用1.5 mm宽的腔室压印了一层PEGDA，铜绿假单胞菌生物膜在其中生长，分泌高丝氨酸内酯（HSL，群体感应的小分子调节剂），并形成这些化合物的空间和时间梯度。在静态生长条件下（即无流动），新生生物膜分泌N-（3-氧代十二烷酰基）HSL，其在水凝胶中形成梯度，并被≤ 8 mm远的铜绿假单胞菌细胞检测到。扩散HSL增加了距离生物膜< 3 mm的群落中细胞的生长速率，其中HSL浓度> 1 µM，并且对更远的群落几乎没有影响。HSL梯度对生物膜结构没有明显的影响。令人惊讶的是，0.1-10 μM的N-（3-氧代十二烷酰基）HSL对液体培养物中的细胞生长没有影响。结果表明，从生物膜中分泌的HSL增强了与表面接触的相邻细胞生长成群落，并可能影响它们的组成、组织和多样性。
This manuscript describes the fabrication of arrays of spatially confined chambers embossed in a layer of poly(ethylene glycol) diacrylate (PEGDA) and their application to studying quorum sensing between communities of Pseudomonas aeruginosa. We hypothesized that biofilms may produce stable chemical signaling gradients in close proximity to surfaces, which influence the growth and development of nearby microcolonies into biofilms. To test this hypothesis we embossed a layer of PEGDA with 1.5-mm wide chambers in which P. aeruginosa biofilms grew, secreted homoserine lactones (HSLs, small molecule regulators of quorum sensing), and formed spatial and temporal gradients of these compounds. In static growth conditions (i.e. no flow), nascent biofilms secreted N-(3-oxododecanoyl) HSL that formed a gradient in the hydrogel and was detected by P. aeruginosa cells that were ≤ 8 mm away. Diffusing HSLs increased the growth rate of cells in communities that were < 3 mm away from the biofilm, where the concentration of HSL was > 1 µM, and had little effect on communities farther away. The HSL gradient had no observable influence on biofilm structure. Surprisingly, 0.1–10 µM of N-(3-oxododecanoyl) HSL had no effect on cell growth in liquid culture. The results suggest that the secretion of HSLs from a biofilm enhances the growth of neighboring cells in contact with surfaces into communities and may influence their composition, organization, and diversity.
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影响因子：
--
作者：
D'Onofrio A;Crawford JM;Stewart EJ;Witt K;Gavrish E;Epstein S;Clardy J;Lewis K
通讯作者：
Lewis K
影响因子：
14.8
作者：
Carnes EC;Lopez DM;Donegan NP;Cheung A;Gresham H;Timmins GS;Brinker CJ
通讯作者：
Brinker CJ
影响因子：
6.2
作者：
Ekblad, Tobias;Bergstroem, Gunnar;Liedberg, Bo
通讯作者：
Liedberg, Bo
影响因子：
3
作者：
COSTERTON, JW;GEESEY, GG;CHENG, KJ
通讯作者：
CHENG, KJ
影响因子：
4.3
作者：
Horswill, Alexander R.;Stoodley, Paul;Stewart, Philip S.;Parsek, Matthew R.
通讯作者：
Parsek, Matthew R.