Identification and Characterization of Two Novel Noda-like Viruses from Rice Plants Showing the Dwarfing Symptom.
Identification and Characterization of Two Novel Noda-like Viruses from Rice Plants Showing the Dwarfing Symptom.
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DOI：
10.3390/v14061159
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发表时间：
2022-05-27
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影响因子：
--
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诺达病毒是小型二分RNA病毒，被认为是动物病毒。在这里，我们通过RNA-seq在田间收集的表现出矮化表型的水稻植株中鉴定了两种新型的节瘤样病毒（称为水稻相关节瘤样病毒1（RNLV 1）和水稻相关节瘤样病毒2（RNLV 2））。RNLV 1基因组由3335 nt RNA 1和1769 nt RNA 2组成，RNLV 2基因组由3279 nt RNA 1和1525 nt RNA 2组成。在两种新病毒的每个基因组中鉴定出三个保守的ORF，分别编码RNA依赖的RNA聚合酶、RNA沉默抑制子和衣壳蛋白。序列比对、蛋白质结构域预测和进化分析结果表明，这两种新病毒与已知的野田病毒，特别是CP有明显的不同。我们还确定了由两种新的节田样病毒编码的B2蛋白可以抑制植物中的RNA沉默。构建了两个反向遗传系统，并用于证明RNLV 1 RNA 1可以在植物细胞中复制，RNLV 1可以在昆虫Sf 9细胞中复制。我们还发现RNLV 1 CP中有两个不寻常的肽酶家族A21结构域，并且RNLV 1 CP可以在酸性环境中自我切割。这些发现为新型野田病毒提供了新的知识。
Nodaviruses are small bipartite RNA viruses and are considered animal viruses. Here, we identified two novel noda-like viruses (referred to as rice-associated noda-like virus 1 (RNLV1) and rice-associated noda-like virus 2 (RNLV2)) in field-collected rice plants showing a dwarfing phenotype through RNA-seq. RNLV1 genome consists of 3335 nt RNA1 and 1769 nt RNA2, and RNLV2 genome consists of 3279 nt RNA1 and 1525 nt RNA2. Three conserved ORFs were identified in each genome of the two novel viruses, encoding an RNA-dependent RNA polymerase, an RNA silencing suppressor, and a capsid protein, respectively. The results of sequence alignment, protein domain prediction, and evolutionary analysis indicate that these two novel viruses are clearly different from the known nodaviruses, especially the CPs. We have also determined that the B2 protein encoded by the two new noda-like viruses can suppress RNA silencing in plants. Two reverse genetic systems were constructed and used to show that RNLV1 RNA1 can replicate in plant cells and RNLV1 can replicate in insect Sf9 cells. We have also found two unusual peptidase family A21 domains in the RNLV1 CP, and RNLV1 CP can self-cleave in acidic environments. These findings provide new knowledge of novel nodaviruses.
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DOI：
10.3390/v13010073
发表时间：
2021-01-07
期刊：
Viruses
影响因子：
--
作者：
Yang C;Wang L;Schwartz K;Burrough E;Groeltz-Thrush J;Chen Q;Zheng Y;Shen H;Li G
通讯作者：
Li G
DOI：
10.1073/pnas.081288198
发表时间：
2001-04-24
影响因子：
11.1
作者：
Dasgupta, R;Garcia, BH;Goodman, RM
通讯作者：
Goodman, RM
影响因子：
5.4
作者：
Franz, Carl J.;Zhao, Guoyan;Wang, David
通讯作者：
Wang, David
影响因子：
3.7
作者：
Dacheux L;Cervantes-Gonzalez M;Guigon G;Thiberge JM;Vandenbogaert M;Maufrais C;Caro V;Bourhy H
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Bourhy H
影响因子：
9.8
作者：
Félix MA;Ashe A;Piffaretti J;Wu G;Nuez I;Bélicard T;Jiang Y;Zhao G;Franz CJ;Goldstein LD;Sanroman M;Miska EA;Wang D
通讯作者：
Wang D