Concentration-Dependent Multi-Potentiality of L-Arginine: Antimicrobial Effect, Hydroxyapatite Stability, and MMPs Inhibition.
Concentration-Dependent Multi-Potentiality of L-Arginine: Antimicrobial Effect, Hydroxyapatite Stability, and MMPs Inhibition.
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L-精氨酸的浓度依赖性多电位：抗菌作用，羟基磷灰石稳定性和MMP抑制。
DOI：
10.3390/molecules26216605
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发表时间：
2021-10-31
期刊：
影响因子：
--
通讯作者：
Daood U
中科院分区：
文献类型：
--
作者：
Bijle MN;Pichika MR;Mak KK;Parolia A;Babar MG;Yiu C;Daood U
本研究旨在探讨L-精氨酸(L-精氨酸)对单种生物膜(变形链球菌/血链球菌)生长及釉质基质的影响。实验组分别为1%、2%、4%精氨酸，以0.9%氯化钠为载体对照。灭菌后的釉质块用试验液和变形链球菌/血链球菌接种在BHI中进行7天的处理。处理后，对处理后的生物膜进行活/死细菌细胞染色，用共聚焦显微镜进行分析。用X射线衍射仪(XRD)、拉曼光谱(RS)和透射电子显微镜(TEM)对釉质样品进行了分析。通过计算分子对接和基质金属蛋白酶测定确定精氨酸与基质金属蛋白酶-2/基质金属蛋白酶-9的分子相互作用。随着精氨酸浓度的增加，细菌存活率显著降低(p<；0.05)。添加1%/2%Arg的样品的X射线衍射峰强度显著高于添加4%Arg的样品和对照组(p<；0.05)。与其他组相比，1%/2%Arg组的RS与矿物相相关的条带显著增加(p<；0.05)。透射电子显微镜分析表明，4%Arg的釉质晶体形状不清。Arg分子与MMPs的对接似乎是可行的，Arg可以抑制MMP2/MMP9(p<；0.05)。L精氨酸补充剂对单种生物膜有一定的抑菌作用。L-精氨酸在较低浓度(1%/2%)时表现出牙釉质羟基磷灰石的稳定性，而该分子具有抑制基质金属蛋白酶-2/基质金属蛋白酶-9的潜能。
This study’s objective was to examine L-arginine (L-arg) supplementation’s effect on mono-species biofilm (Streptococcus mutans/Streptococcus sanguinis) growth and underlying enamel substrates. The experimental groups were 1%, 2%, and 4% arg, and 0.9% NaCl was used as the vehicle control. Sterilised enamel blocks were subjected to 7-day treatment with test solutions and S. mutans/S. sanguinis inoculum in BHI. Post-treatment, the treated biofilms stained for live/dead bacterial cells were analysed using confocal microscopy. The enamel specimens were analysed using X-ray diffraction crystallography (XRD), Raman spectroscopy (RS), and transmission electron microscopy (TEM). The molecular interactions between arg and MMP-2/MMP-9 were determined by computational molecular docking and MMP assays. With increasing arg concentrations, bacterial survival significantly decreased (p < 0.05). The XRD peak intensity with 1%/2% arg was significantly higher than with 4% arg and the control (p < 0.05). The bands associated with the mineral phase by RS were significantly accentuated in the 1%/2% arg specimens compared to in other groups (p < 0.05). The TEM analysis revealed that 4% arg exhibited an ill-defined shape of enamel crystals. Docking of arg molecules to MMPs appears feasible, with arg inhibiting MMP-2/MMP-9 (p < 0.05). L-arginine supplementation has an antimicrobial effect on mono-species biofilm. L-arginine treatment at lower (1%/2%) concentrations exhibits enamel hydroxyapatite stability, while the molecule has the potential to inhibit MMP-2/MMP-9.
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影响因子：
3.7
作者：
Nascimento MM;Browngardt C;Xiaohui X;Klepac-Ceraj V;Paster BJ;Burne RA
通讯作者：
Burne RA
影响因子：
7.6
作者：
Kato, M. T.;Bolanho, A.;Buzalaf, M. A. R.
通讯作者：
Buzalaf, M. A. R.
影响因子：
4.2
作者：
Ganss, C.;Lussi, A.;Schlueter, N.
通讯作者：
Schlueter, N.
影响因子：
4.4
作者：
Bijle, Mohammed Nadeem;Abdalla, Mohamed Mahmoud;Yiu, Cynthia Kar Yung
通讯作者：
Yiu, Cynthia Kar Yung
DOI：
10.1007/s10096-013-1993-7
发表时间：
2014-04
影响因子：
4.5
作者：
Krzysciak, W.;Jurczak, A.;Koscielniak, D.;Bystrowska, B.;Skalniak, A.
通讯作者：
Skalniak, A.