Comparison of oxidative properties, light absorbance, total and elemental mass concentration of ambient PM2.5 collected at 20 European sites.
Comparison of oxidative properties, light absorbance, total and elemental mass concentration of ambient PM2.5 collected at 20 European sites.
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DOI：
10.1289/ehp.8584
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发表时间：
2006-05
影响因子：
10.4
通讯作者：
Borm PJ
中科院分区：
文献类型：
--
作者：
Künzli N;Mudway IS;Götschi T;Shi T;Kelly FJ;Cook S;Burney P;Forsberg B;Gauderman JW;Hazenkamp ME;Heinrich J;Jarvis D;Norbäck D;Payo-Losa F;Poli A;Sunyer J;Borm PJ
有人提出，颗粒的氧化还原活性可能是其毒性的一个主要决定因素。我们测量了环境中的细颗粒[空气动力学直径为PM2.5的颗粒物≤2.5μm(PM2.5)]在氧化剂环境中形成羟基自由基(·OH)的能力，以及在呼吸道衬里液体的自然还原环境中消耗生理性抗氧化剂(抗坏血酸、谷胱甘肽)的能力。研究的目的是检验这些毒理学相关指标与其他PM特征的关系，如总质量浓度和元素质量浓度以及光吸光度。参与欧洲共同体呼吸健康调查的20个中心提供了一年多来收集的重量PM2.5样本(n=716)。用反射法测量了滤光片的光吸收。在稀释到标准浓度之前，通过涡流和超声波从过滤器中回收PM悬浮液。然后，通过测量它们在过氧化氢存在下产生·OH的能力，使用电子自旋共振和5，5-二甲基-1-吡咯啉-N-氧化物作为自旋陷阱，或通过建立它们从RTLF合成模型中耗尽抗氧化剂的能力，来评估这些颗粒悬浮液的氧化活性。PM的氧化活性在欧洲不同的采样点之间差异很大。氧化活性与PM的所有其他特征之间的相关性很低，无论是在中心内(时间相关性)还是跨社区(年度平均值)。因此，不能确定PM氧化还原活性的单一替代测量。由于这些新的措施被建议反映PM的关键生物学机制，它们的使用可能在流行病学研究中具有相关性。因此，确定合适的方法来测定PM的氧化活性是很重要的
It has been proposed that the redox activity of particles may represent a major determinant of their toxicity. We measured the in vitro ability of ambient fine particles [particulate matter with aerodynamic diameters ≤2.5 μm (PM2.5)] to form hydroxyl radicals (•OH) in an oxidant environment, as well as to deplete physiologic antioxidants (ascorbic acid, glutathione) in the naturally reducing environment of the respiratory tract lining fluid (RTLF). The objective was to examine how these toxicologically relevant measures were related to other PM characteristics, such as total and elemental mass concentration and light absorbance. Gravimetric PM2.5 samples (n = 716) collected over 1 year from 20 centers participating in the European Community Respiratory Health Survey were available. Light absorbance of these filters was measured with reflectometry. PM suspensions were recovered from filters by vortexing and sonication before dilution to a standard concentration. The oxidative activity of these particle suspensions was then assessed by measuring their ability to generate •OH in the presence of hydrogen peroxide, using electron spin resonance and 5,5-dimethyl-1-pyrroline-N-oxide as spin trap, or by establishing their capacity to deplete antioxidants from a synthetic model of the RTLF. PM oxidative activity varied significantly among European sampling sites. Correlations between oxidative activity and all other characteristics of PM were low, both within centers (temporal correlation) and across communities (annual mean). Thus, no single surrogate measure of PM redox activity could be identified. Because these novel measures are suggested to reflect crucial biologic mechanisms of PM, their use may be pertinent in epidemiologic studies. Therefore, it is important to define the appropriate methods to determine oxidative activity of PM.
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影响因子：
5
作者：
Hazenkamp-Von Arx, ME;Götschi, T;Künzli, N
通讯作者：
Künzli, N
影响因子：
10.4
作者：
Gilliland, FD;McConnell, R;Gong, H
通讯作者：
Gong, H
DOI：
10.1152/ajplung.00078.2004
发表时间：
2004-10-01
影响因子：
4.9
作者：
Heckel, K;Kiefmann, R;Goetz, AE
通讯作者：
Goetz, AE
影响因子：
24.3
作者：
Jarvis, D
通讯作者：
Jarvis, D
影响因子：
5
作者：
Götschi, T;Hazenkamp-Von Arxb, ME;Künzli, N
通讯作者：
Künzli, N