Serpentine Ultralong Path with Extended Routing (SUPER) High Resolution Traveling Wave Ion Mobility-MS using Structures for Lossless Ion Manipulations.
Serpentine Ultralong Path with Extended Routing (SUPER) High Resolution Traveling Wave Ion Mobility-MS using Structures for Lossless Ion Manipulations.
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DOI：
10.1021/acs.analchem.7b00185
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发表时间：
2017-04-18
影响因子：
7.4
通讯作者：
Smith RD
中科院分区：
文献类型：
--
作者：
Deng L;Webb IK;Garimella SVB;Hamid AM;Zheng X;Norheim RV;Prost SA;Anderson GA;Sandoval JA;Baker ES;Ibrahim YM;Smith RD
离子淌度 (IM) 分离具有广泛的分析应用，但分辨率不足往往限制其实用性。在这里，我们报告了无损离子操纵（SLIM）蛇形超长路径结构中的离子淌度分离，具有扩展路由（SUPER）行波（TW）离子淌度（IM）模块与质谱（MS）相结合。离子被射频场和直流保护偏压限制在 SLIM 中，从而在大大延长的路径上实现基本上无损的 TW 传输。扩展路由利用了多次通过（例如，通过 13.5 m 蛇形路径的 81 次路径，约 1094 m），并通过引入无损离子开关来促进，该开关允许离子被引导至 MS 检测器或通过蛇形分离区域进行另一次通过，从而理论上允许无限的 IM 路径长度。多通道 SUPER IM-MS 提供的分辨率与通道数（或总路径长度）的平方根大致成正比。与市售漂移管 IM 和其他基于 TWIM 的平台相比，安捷伦调谐混合物 m/z 622 和 922 离子在 40 次通过中实现了 30 倍以上的 IM 分辨率（~340 与~10）。对异构糖乳-N-六糖和乳-N-新六糖的初步评估显示异构体结构已得到基线解析，并且在9次通过后揭示了乳-N-新六糖的新构象特征。新的 SLIM 超高分辨率 TWIM 平台与 MS 结合具有广泛的实用性，预计将能够实现广泛的以前具有挑战性或棘手的分离。
Ion mobility (IM) separations have a broad range of analytical applications, but insufficient resolution often limits their utility. Here, we report on ion mobility separations in a structures for lossless ion manipulations (SLIM) serpentine ultralong path with extended routing (SUPER) traveling wave (TW) ion mobility (IM) module in conjunction with mass spectrometry (MS). Ions were confined in the SLIM by rf fields in conjunction with a DC guard bias, enabling essentially lossless TW transmission over greatly extended paths. The extended routing utilized multiple passes (e.g., ~1094 m over 81 passes through the 13.5 m serpentine path) and was facilitated by the introduction of a lossless ion switch that allowed ions to be directed to either the MS detector or for another pass through the serpentine separation region, allowing theoretically unlimited IM path lengths. The multipass SUPER IM-MS provided resolution approximately proportional to the square root of the number of passes (or total path length). More than 30-fold higher IM resolution (~340 vs ~10) for Agilent tuning mix m/z 622 and 922 ions was achieved for 40 passes compared to commercially available drift tube IM and other TWIM-based platforms. An initial evaluation of the isomeric sugars lacto-N-hexaose and lacto-N-neohexaose showed the isomeric structures to be baseline resolved, and a new conformational feature for lacto-N-neohexaose was revealed after 9 passes. The new SLIM SUPER high resolution TWIM platform has broad utility in conjunction with MS and is expected to enable a broad range of previously challenging or intractable separations.
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DOI：
10.1007/s13361-014-0976-y
发表时间：
2014-11
影响因子：
3.2
作者：
Garimella, Sandilya V. B.;Ibrahim, Yehia M.;Webb, Ian K.;Tolmachev, Aleksey V.;Zhang, Xinyu;Prost, Spencer A.;Anderson, Gordon A.;Smith, Richard D.
通讯作者：
Smith, Richard D.
影响因子：
7.4
作者：
Merenbloom, Samuel I.;Glaskin, Rebecca S.;Henson, Zachary B.;Clemmer, David E.
通讯作者：
Clemmer, David E.
影响因子：
2
作者：
Giles, Kevin;Williams, Jonathan P.;Campuzano, Iain
通讯作者：
Campuzano, Iain
影响因子：
2
作者：
Dear, Gordon J.;Munoz-Muriedas, Jordi;Campuzano, Iain
通讯作者：
Campuzano, Iain
影响因子：
7.4
作者：
Glaskin, Rebecca S.;Valentine, Stephen J.;Clemmer, David E.
通讯作者：
Clemmer, David E.