Maggot Extract Interrupts Bacterial Biofilm Formation and Maturation in Combination with Antibiotics by Reducing the Expression of Virulence Genes.
Maggot Extract Interrupts Bacterial Biofilm Formation and Maturation in Combination with Antibiotics by Reducing the Expression of Virulence Genes.
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DOI：
10.3390/life12020237
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发表时间：
2022-02-04
期刊：
影响因子：
--
通讯作者：
Behr B
中科院分区：
文献类型：
--
作者：
Becerikli M;Wallner C;Dadras M;Wagner JM;Dittfeld S;Jettkant B;Gestmann F;Mehlhorn H;Mehlhorn-Diehl T;Lehnhardt M;Behr B
生物膜是包裹在细胞外聚合物基质中的细菌聚集体，其充当保护微生物群落的扩散屏障。细菌通讯是通过一种称为群体感应（quorum sensing，QS）因子的小信号分子进行的，它参与了毒力基因的激活和生物膜的形成。绿色瓶绿蝇的幼虫通过机械作用（清创）和蛋白水解消化去除坏死组织。我们从幼虫提取物中制备了一种冷冻干燥的可储存粉末，并研究了其对生物膜的治疗作用。浓度为6和12 mg/mL的幼虫提取物与0.5%抗生素（约50 U/mL青霉素和50 μg/mL链霉素）联合使用，可减少自由漂浮（无张力）铜绿假单胞菌的维持，但对金黄色葡萄球菌无影响，对皮肤细胞无毒性。我们建立了离体人皮肤创伤模型。在抗生素（0.5%）存在下，浓度为24和75 mg/mL的幼虫提取物显著破坏了铜绿假单胞菌和S.金黄色葡萄球菌生物膜此外，SEM分析揭示了铜绿假单胞菌上的裂纹和间隙形成。在用幼虫提取物与抗生素组合处理后，观察到铜绿假单胞菌生物膜成熟和毒力基因（lasR、rhlR和rhlA）的生物膜表面和降低的表达。
Biofilms are aggregates of bacteria encased in an extracellular polymer matrix that acts as a diffusion barrier protecting the microbial community. Bacterial communication occurs by small signaling molecules called quorum sensing (QS) factors, which are involved in the activation of virulence genes and formation of biofilms. Larvae of the green bottle blowfly Lucilia sericata remove necrotic tissue by mechanical action (debridement) and proteolytic digestion. We produced a freeze-dried storable powder from larval extract and investigated its therapeutic effect on biofilms. Larval extract in concentrations of 6 and 12 mg/mL in combination with 0.5% antibiotics (≙50 U/mL penicillin and 50 μg/mL streptomycin) diminished free-floating (planktonic) Pseudomonas aeruginosa maintenance, while it showed no effect on Staphylococcus aureus and was not toxic to dermal cells. We established an ex vivo human dermal wound model. Larval extract in concentrations of 24 and 75 mg/mL in the presence of antibiotics (0.5%) significantly destroyed the biofilm stability of both P. aeruginosa and S. aureus biofilms. Furthermore, SEM analyses revealed crack and gap formations on P. aeruginosa. biofilm surface and decreased expression of P. aeruginosa biofilm maturation and virulence genes (lasR, rhlR and rhlA) was observed after treatment by larval extract in combination with antibiotics.
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影响因子：
3.7
作者：
Becerikli, Mustafa;Jaurich, Henriette;Behr, Bjoern
通讯作者：
Behr, Bjoern
影响因子：
3.7
作者：
Dalton T;Dowd SE;Wolcott RD;Sun Y;Watters C;Griswold JA;Rumbaugh KP
通讯作者：
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影响因子：
3.8
作者：
Shimada, I
通讯作者：
Shimada, I
影响因子：
64.8
作者：
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通讯作者：
O'Toole, GA
影响因子：
5.7
作者：
Moradali MF;Ghods S;Rehm BH
通讯作者：
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