Phospholipid-sensitive Ca2+-dependent protein kinase and its substrates in human neutrophils.
Phospholipid-sensitive Ca2+-dependent protein kinase and its substrates in human neutrophils.
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人中性粒细胞中磷脂敏感的 Ca2 依赖性蛋白激酶及其底物。
DOI：
10.1016/0006-291x(83)91376-1
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发表时间：
1983
影响因子：
3.1
通讯作者：
Kuo,JF
中科院分区：
文献类型：
--
作者：
Helfman,DM;Appelbaum,BD;Vogler,WR;Kuo,JF
Abstract Phospholipid-sensitive Ca 2+-dependent protein kinase (PL-Ca-PK) was found to be present at a high level in human neutrophils, with its activity localized in the particulate fraction. In contrast, cyclic AMP-dependent protein kinase (A-PK) and cyclic GMP-dependent protein kinase (G-PK), present at lower levels compared to PL-Ca-PK, were localized in the cytosolic fraction. Phosphorylation of several endogenous proteins (mol. wts. 89,000, 38,000, 34,000, 17,000 and 15,000), also localized in the particulate fraction, was stimulated specifically by a combination of phosphatidylserine and Ca 2+, whereas no substrate proteins were observed for the calmodulin-sensitive Ca 2+-dependent protein kinase system under the same incubation conditions. Although no substrate proteins for G-PK were detected, one substrate (mol. wt. 19,000) for A-PK was observed. Phosphorylation of substrates for PL-Ca-PK, but not that for A-PK and for enzymes independent of Ca 2+ or cyclic AMP, was inhibited by a variety of agents, including trifluoperazine, W-7 [N-(6-aminohexyl)-5-chloro-1-naphthalene-sulfonamide], adriamycin, palmitoylcarnitine, and melittin. The present findings suggest that the phospholipid Ca 2+-stimulated protein phosphorylation system may be important in the membrane associated functions of human neutrophils.
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影响因子：
7.8
作者：
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DOI：
10.1083/jcb.62.3.594
发表时间：
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期刊：
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影响因子：
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发表时间：
1979-01-01
影响因子：
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DOI：
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发表时间：
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Biochimica et biophysica acta
影响因子：
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作者：
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