A deficiency screen for zygotic loci required for establishment and patterning of the epidermis in Caenorhabditis elegans.
A deficiency screen for zygotic loci required for establishment and patterning of the epidermis in Caenorhabditis elegans.
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秀丽隐杆线虫表皮建立和图案化所需的合子位点的缺陷筛选。
DOI：
10.1093/genetics/146.1.185
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发表时间：
1997
期刊：
影响因子：
3.3
通讯作者：
Rothman,JH
中科院分区：
文献类型：
--
作者：
Terns,RM;Kroll-Conner,P;Zhu,J;Chung,S;Rothman,JH
为了确定表皮的建立和模式化所需的基因组区域，我们筛选了58个缺陷，这些缺陷共同缺失了至少67%的秀丽隐杆线虫基因组。缺陷纯合子胚胎的表皮模式进行了分析，通过检查的三个主要的表皮细胞类型之一，缝细胞特异性标记的表达。表皮和内部器官的组织也进行了分析，使用上皮粘附连接特异性的单克隆抗体。虽然7种缺陷对缝细胞的产生没有明显影响，但发现21种缺陷导致缝细胞数量低于正常水平，5种缺陷导致缝细胞数量过多。另外23个缺陷阻断了接缝细胞标记物的表达，在某些情况下没有阻止细胞增殖。两种缺陷导致多核缝细胞。还鉴定了导致表皮细胞数量低于正常、表皮细胞过度融合成大合胞体或异常表皮分化的缺陷。最后，对内部上皮细胞的分析揭示了导致内脏器官形成缺陷的缺陷，包括肠的循环和咽腔的分叉。本研究揭示了C.表皮和其他上皮细胞的形成需要合子基因组。
To identify genomic regions required for establishment and patterning of the epidermis, we screened 58 deficiencies that collectively delete at least ∼67% of theCaenorhabditis elegansgenome. The epidermal pattern of deficiency homozygous embryos was analyzed by examining expression of a marker specific for one of the three major epidermal cell types, the seam cells. The organization of the epidermis and internal organs was also analyzed using a monoclonal antibody specific for epithelial adherens junctions. While seven deficiencies had no apparent effect on seam cell production, 21 were found to result in subnormal, and five in excess numbers of these cells. An additional 23 deficiencies blocked expression of the seam cell marker, in some cases without preventing cell proliferation. Two deficiencies result in multinucleate seam cells. Deficiencies were also identified that result in subnormal numbers of epidermal cells, hyperfusion of epidermal cells into a large syncytium, or aberrant epidermal differentiation. Finally, analysis of internal epithelia revealed deficiencies that cause defects in formation of internal organs, including circularization of the intestine and bifurcation of the pharynx lumen. This study reveals that many regions of theC. elegansgenome are required zygotically for patterning of the epidermis and other epithelia.
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细胞相互作用参与早期秀丽隐杆线虫胚胎的测定
影响因子：
2.6
作者：
R. Schnabel
通讯作者：
R. Schnabel
glp-1（秀丽隐杆线虫发育过程中细胞通讯所需的基因）的抑制因子定义了一组相互作用的基因。
影响因子：
3.3
作者：
Maine,EM;Kimble,J
通讯作者：
Kimble,J
秀丽隐杆线虫胚胎发生过程中体壁肌肉发育所需遗传位点的筛选。
影响因子：
3.3
作者：
Ahnn,J;Fire,A
通讯作者：
Fire,A
影响因子：
4.6
作者：
M. Labouesse;S. Sookhareea;H. Horvitz
通讯作者：
M. Labouesse;S. Sookhareea;H. Horvitz
秀丽隐杆线虫的 unc-22(IV) 区域：致死突变的遗传分析。
影响因子：
3.3
作者：
Denise V. Clark;T. Rogalski;Leo M. Donati;David L. Baillie
通讯作者：
David L. Baillie