Homology of the NH2-terminal amino acid sequences of the heavy and light chains of human monoclonal lupus autoantibodies containing the dominant 16/6 idiotype.
Homology of the NH2-terminal amino acid sequences of the heavy and light chains of human monoclonal lupus autoantibodies containing the dominant 16/6 idiotype.
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含有显性 16/6 独特型的人单克隆狼疮自身抗体的重链和轻链 NH2 末端氨基酸序列的同源性。
DOI：
10.1172/jci111808
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Furie,B
中科院分区：
文献类型：
--
作者：
Atkinson,PM;Lampman,GW;Furie,BC;Naparstek,Y;Schwartz,RS;Stollar,BD;Furie,B
从两例系统性红斑狼疮患者淋巴细胞制备的人-人杂交瘤产生的5株抗DNA单抗的重链和轻链，用自动Edman降解方法测定了NH2末端的氨基酸序列。其中4个抗体与独特型系统16/6密切相关，而第5个抗体与独特型无关。16/6独特型阳性自身抗体(HF2-1/13b、HF2-1/17、HF2-18/2和HF3-16/6)轻链的氨基酸序列从第1位到第40位完全相同。它们的骨架结构具有VKI轻链的特征。16/6独特型阴性自身抗体HF6-21/28的轻链是VKII亚群特有的。16/6独特型阳性自身抗体重链的氨基酸序列从第1位到第40位几乎相同。骨架结构具有VHIII亚群的特征。相反，杂交瘤的GM4672融合伙伴产生了少量带有VHI重链和VKI轻链的免疫球蛋白。狼疮自身抗体的重链和轻链与16/6系统的独特型相关，与WEA有显著的序列同源性，WEA是一种与克雷伯氏菌多糖结合并表达16/6独特型的Waldenstrom IgM。这些结果表明，所研究的狼疮自身抗体的重链和轻链的氨基末端具有惊人的同源性，提示16/6独特型阳性DNA结合狼疮自身抗体的重链和轻链的V区各由一个生殖系基因编码。
The NH2-terminal amino acid sequences have been determined by automated Edman degradation for the heavy and light chains of five monoclonal IgM anti-DNA autoantibodies that were produced by human-human hybridomas derived from lymphocytes of two patients with systemic lupus erythematosus. Four of the antibodies were closely related to the idiotype system 16/6, whereas the fifth antibody was unrelated idiotypically. The light chains of the 16/6 idiotype-positive autoantibodies (HF2-1/13b, HF2-1/17, HF2-18/2, and HF3-16/6) had identical amino acid sequences from residues 1 to 40. Their framework structures were characteristic of VKI light chains. The light chain of the 16/6 idiotype-negative autoantibody HF6-21/28 was characteristic of the VKII subgroup. The heavy chains of the 16/6 idiotype-positive autoantibodies had nearly identical amino acid sequences from residues 1 to 40. The framework structures were characteristic of the VHIII subgroup. In contrast, the GM4672 fusion partner of the hybridoma produced small quantities of an IgG with a VHI heavy chain and a VKI light chain. The heavy chains of the lupus autoantibodies and the light chains of those autoantibodies that were idiotypically related to the 16/6 system had marked sequence homology with WEA, a Waldenstrom IgM that binds to Klebsiella polysaccharides and expresses the 16/6 idiotype. These results indicate a striking homology in the amino termini of the heavy and light chains of the lupus autoantibodies studied and suggest that the V regions of the heavy and light chains of the 16/6 idiotype-positive DNA-binding lupus auto-antibodies are each encoded by a single germ line gene.
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人单克隆 IgM（蛋白质 WEA）Fv 区的氨基酸序列，具有针对克雷伯氏菌多糖 K30 和 K33 中 3,4-丙酮酰化半乳糖的抗体活性。
DOI：
10.1073/pnas.80.15.4837
发表时间：
1983
影响因子：
11.1
作者：
Goñi,F;Frangione,B
通讯作者：
Frangione,B
人-人杂交瘤产生的单克隆狼疮自身抗体的多特异性。
DOI：
10.1056/nejm198302243080802
发表时间：
1983
期刊：
The New England journal of medicine
影响因子：
--
作者：
Shoenfeld,Y;Rauch,J;Massicotte,H;Datta,SK;Andre-Schwartz,J;Stollar,BD;Schwartz,RS
通讯作者：
Schwartz,RS
针对 NZB/NZW F1 小鼠 DNA 和 RNA 的单克隆抗体：抗原特异性和 NH2 末端氨基酸序列。
影响因子：
4.4
作者：
D. Eilat;M. Hochberg;J. Pumphrey;S. Rudikoff
通讯作者：
S. Rudikoff
杂交瘤 DNA 自身抗体之间的抗原结合多样性和独特型交叉反应。
影响因子：
4.4
作者：
Chester Andrzejewski;J. Rauch;Eileen M. Lafer;B. Stollar;Robert S. Schwartz
通讯作者：
Robert S. Schwartz
DNA 杂交瘤自身抗体。
影响因子：
4.4
作者：
C. Andrzejewski;B. Stollar;T. Lalor;R. Schwartz
通讯作者：
R. Schwartz