Multilocus Sequence Typing and Antifungal Susceptibility of Vaginal and Non-vaginal Candida glabrata Isolates From China.
Multilocus Sequence Typing and Antifungal Susceptibility of Vaginal and Non-vaginal Candida glabrata Isolates From China.
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中国阴道和非阴道光滑念珠菌的多位点序列分型及抗真菌敏感性
DOI：
10.3389/fmicb.2022.808890
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发表时间：
2022
影响因子：
5.2
通讯作者：
Ying C
中科院分区：
文献类型：
--
作者：
Chen Y;Wu Y;Lulou K;Yao D;Ying C
光滑念珠菌是念珠菌感染的常见原因。在本研究中，我们调查了阴道和非阴道念珠菌的抗真菌药物敏感性和分子流行病学。光果苔属分离物76个阴道C从外阴阴道念珠菌病患者和57例非阴道念珠菌分离的光滑念珠菌菌株。在中国上海的两家医院收集光滑念珠菌分离株。采用微量肉汤稀释法检测药物敏感性。多位点序列分型用于基因分型。总的来说，28（21.1%），28（21.1%）和29（21.8%）C。光滑型分离株分别对氟康唑、伊曲康唑和伏立康唑耐药。简言之，18例（23.7%）、18例（23.7%）和19例（25%）阴道菌株对氟康唑、伊曲康唑和伏立康唑耐药。非阴道感染菌株对上述药物的耐药率均为17.5%（10/57）。所有分离株均保留对阿替霉素B的敏感性，只有4株非阴道分离株对卡泊芬净耐药。基因分型确定了17种ST模式。在非阴道样品中，除了一种基因型（ST-182）之外，出现与阴道样品中相同的基因型，而在阴道样品中出现更多的基因型（ST 8、ST 19、ST 45、ST 55、ST 66、ST 80、ST 138和ST 17）。最常见的基因型为ST 7（81株），其次为ST 10（14株）和ST 15（11株）。大多数耐药表型菌株（25/30，83.3%）与优势基因型（ST 7）相关，其余为ST 3（2/30，6.7%）、ST 10（1/30，3.3%）、ST 19（1/30，3.3%）和ST 45（1/30，3.3%）。我们的调查显示，阴道和非阴道C。光果苔属分离物此外，没有与抗性表型相关的基因型。
Candida glabrata is a common cause of Candida infections. In our present study, we investigated the antifungal susceptibility and molecular epidemiology of vaginal and non-vaginal C. glabrata isolates. Seventy-six vaginal C. glabrata strains isolated from patients with vulvovaginal candidiasis and 57 non-vaginal C. glabrata isolates were collected at two hospitals in Shanghai, China. Antifungal susceptibility was examined using a broth microdilution method. Multilocus sequence typing was used for genotyping. Overall, 28 (21.1%), 28 (21.1%), and 29 (21.8%) C. glabrata isolates were resistant to fluconazole, itraconazole, and voriconazole, respectively. Briefly, 18 (23.7%), 18 (23.7%), and 19 (25%) vaginal strains were resistant to fluconazole, itraconazole, and voriconazole. While the resistance to these antifungals were all 17.5% (10/57) in non-vaginal strains. All isolates retained susceptibility to amphotericin B, and only four non-vaginal isolates were caspofungin resistant. Genotyping identified 17 ST patterns. In non-vaginal samples, the same genotypes appear as in the vaginal samples, except for one genotype (ST-182), while in the vaginal samples more genotypes appear (ST8, ST19, ST45, ST55, ST66, ST80, ST138, and ST17). The most common genotype was ST7 (81 strains), followed by ST10 (14 strains) and ST15 (11 strains). The majority of resistant phenotype strains (25/30, 83.3%) correlated to the predominant genotype (ST7), and the rest belonged to ST3 (2/30, 6.7%), ST10 (1/30, 3.3%), ST19 (1/30, 3.3%), and ST45 (1/30, 3.3%). Our survey revealed cross-resistance in vaginal and non-vaginal C. glabrata isolates. Moreover, there is no genotype associated with the resistance phenotype.
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影响因子：
5.2
作者：
Byun SA;Won EJ;Kim MN;Lee WG;Lee K;Lee HS;Uh Y;Healey KR;Perlin DS;Choi MJ;Kim SH;Shin JH
通讯作者：
Shin JH
影响因子：
2.4
作者：
Darwish, R. M.;AlKawareek, M. Y.;Alkilany, A. M.
通讯作者：
Alkilany, A. M.
影响因子：
5.2
作者：
Song, Yinggai;Chen, Xianlian;Li, Ruoyu
通讯作者：
Li, Ruoyu
影响因子：
2.2
作者：
Canela, Heliara Maria Spina;Cardoso, Barbara;da Silva Ferreira, Marcia Eliana
通讯作者：
da Silva Ferreira, Marcia Eliana
影响因子：
7.1
作者：
Cortegiani A;Misseri G;Fasciana T;Giammanco A;Giarratano A;Chowdhary A
通讯作者：
Chowdhary A