Effect of injection routes on the biodistribution, clearance, and tumor uptake of carbon dots.
Effect of injection routes on the biodistribution, clearance, and tumor uptake of carbon dots.
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DOI：
10.1021/nn401911k
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发表时间：
2013-07-23
期刊：
影响因子：
17.1
通讯作者：
Chen, Xiaoyuan
中科院分区：
文献类型：
--
作者：
Huang, Xinglu;Zhang, Fan;Zhu, Lei;Choi, Ki Young;Guo, Ning;Guo, Jinxia;Tackett, Kenneth;Anilkumar, Parambath;Liu, Gang;Quan, Qimeng;Choi, Hak Soo;Niu, Gang;Sun, Ya-Ping;Lee, Seulki;Chen, Xiaoyuan
光致发光碳基纳米材料的出现在良性纳米探针的发展中显示出令人兴奋的潜力。然而，这些颗粒的体内动力学行为是必要的临床翻译是知之甚少。在这项研究中，荧光碳点（C-dots）的合成和三种注射途径对他们的命运在体内的影响进行了探讨，通过使用近红外荧光（NIRF）和正电子发射断层扫描（PET）成像技术。我们发现，在所有三种注射途径后，C点都能有效且快速地从体内排出。C-dots清除率依次为：静脉注射>肌肉注射>皮下注射。颗粒在网状内皮系统（RES）中的滞留相对较低，并显示出较高的肿瘤背景对比度。此外，不同的注射途径也导致不同的血液清除模式和肿瘤对C-点的摄取。这些结果满足了临床翻译的需要，并应促进进一步研究在临床环境中使用碳基纳米探针的可能性的努力。更广泛地说，我们为纳米平台在各种注射途径下的体内行为提供了测试蓝图，这是朝着纳米颗粒安全性和有效性分析迈出的重要一步。
The emergence of photoluminescent carbon-based nanomaterials has shown exciting potential in the development of benign nanoprobes. However, the in vivo kinetic behaviors of these particles that are necessary for clinical translation are poorly understood to date. In this study, fluorescent carbon dots (C-dots) were synthesized and the effect of three injection routes on their fate in vivo was explored by using both near-infrared fluorescence (NIRF) and positron emission tomography (PET) imaging techniques. We found that C-dots are efficiently and rapidly excreted from the body after all three injection routes. The clearance rate of C-dots is ranked as: intravenous > intramuscular > subcutaneous. The particles had relatively low retention in the reticuloendothelial system (RES) and showed high tumor-to-background contrast. Furthermore, different injection routes also resulted in different blood clearance patterns and tumor uptakes of C-dots. These results satisfy the need for clinical translation and should promote efforts to further investigate the possibility of using carbon-based nanoprobes in a clinical setting. More broadly, we provide a testing blueprint for in vivo behavior of nanoplatforms under various injection routes, an important step forward towards safety and efficacy analysis of nanoparticles.
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影响因子：
17.1
作者：
Kunjachan S;Gremse F;Theek B;Koczera P;Pola R;Pechar M;Etrych T;Ulbrich K;Storm G;Kiessling F;Lammers T
通讯作者：
Lammers T
影响因子：
12.4
作者：
Clift MJ;Stone V
通讯作者：
Stone V
影响因子：
46.9
作者：
Colvin, VL
通讯作者：
Colvin, VL
影响因子：
46.9
作者：
Jaiswal, JK;Mattoussi, H;Simon, SM
通讯作者：
Simon, SM
影响因子：
15
作者：
Liu, Wenhao;Choi, Hak Soo;Bawendi, Moungi
通讯作者：
Bawendi, Moungi