LoSWEET14, a Sugar Transporter in Lily, Is Regulated by Transcription Factor LoABF2 to Participate in the ABA Signaling Pathway and Enhance Tolerance to Multiple Abiotic Stresses in Tobacco.
LoSWEET14, a Sugar Transporter in Lily, Is Regulated by Transcription Factor LoABF2 to Participate in the ABA Signaling Pathway and Enhance Tolerance to Multiple Abiotic Stresses in Tobacco.
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百合中的糖转运蛋白 LoSWEET14 受转录因子 LoABF2 调控，参与 ABA 信号通路并增强烟草对多种非生物胁迫的耐受性
DOI：
10.3390/ijms232315093
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发表时间：
2022-12-01
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
糖的运输和分配在百合鳞茎发育和抗非生物胁迫中起着重要作用。本研究从东方杂交百合“索邦”中鉴定出糖最终输出转运蛋白（SWEET）基因家族成员LoSWEET 14。LoSWEET 14编码278个氨基酸的蛋白质，能够转运蔗糖和某些类型的己糖。LoSWEET 14基因在干旱、低温、盐胁迫和脱落酸（阿坝）处理下的转录水平显著增加。LoSWEET 14在烟草中的过表达表明，与野生型株系相比，转基因株系具有更大的叶片，积累更多的可溶性糖，并且更耐干旱，寒冷和盐胁迫，同时对阿坝更敏感。启动子分析表明，LoSWEET 14启动子中存在多个胁迫相关的顺式作用元件。根据顺式作用元件的分布，构建了不同长度的5′-缺失片段，发现LoSWEET 14-pro3（-540 bp）能够驱动GUS基因在非生物胁迫和阿坝处理下表达。此外，酵母单杂交（Y1 H）试验证明，来自百合的AREB/ABF（ABRE结合蛋白/ABRE结合因子）（LoABF 2）可以与LoSWEET 14的启动子结合。这些发现表明LoSWEET 14被LoABF 2诱导参与阿坝信号通路，促进可溶性糖积累，以响应多种非生物胁迫。
Sugar transport and distribution plays an important role in lily bulb development and resistance to abiotic stresses. In this study, a member of the Sugar Will Eventually be Exported Transporters (SWEET) gene family, LoSWEET14, from Oriental hybrid lily ‘Sorbonne’ was identified. LoSWEET14 encodes a protein of 278 amino acids and is capable of transporting sucrose and some types of hexoses. The transcript level of the LoSWEET14 gene was significantly increased under various stress conditions including drought, cold, salt stresses, and abscisic acid (ABA) treatment. Overexpression of LoSWEET14 in tobacco (Nicotiana tabacum) showed that the transgenic lines had larger leaves, accumulated more soluble sugars, and were more resistant to drought, cold, and salt stresses, while becoming more sensitive to ABA compared with wild-type lines. Promoter analysis revealed that multiple stress-related cis-acting elements were found in the promoter of LoSWEET14. According to the distribution of cis-acting elements, different lengths of 5′-deletion fragments were constructed and the LoSWEET14-pro3(-540 bp) was found to be able to drive GUS gene expression in response to abiotic stresses and ABA treatment. Furthermore, a yeast one hybrid (Y1H) assay proved that the AREB/ABF (ABRE-binding protein/ABRE-binding factor) from lilies (LoABF2) could bind to the promoter of LoSWEET14. These findings indicated that LoSWEET14 is induced by LoABF2 to participate in the ABA signaling pathway to promote soluble sugar accumulation in response to multiple abiotic stresses.
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影响因子：
5.6
作者：
Lim CW;Baek W;Jung J;Kim JH;Lee SC
通讯作者：
Lee SC
影响因子：
5.1
作者：
Dekkers, Bas J. W.;Schuurmans, Jolanda A. M. J.;Smeekens, Sjef C. M.
通讯作者：
Smeekens, Sjef C. M.
影响因子：
3
作者：
Gao, Suping;Zhu, Yuan;Si, Wenhui
通讯作者：
Si, Wenhui
影响因子：
5.6
作者：
Gill RA;Ahmar S;Ali B;Saleem MH;Khan MU;Zhou W;Liu S
通讯作者：
Liu S
影响因子：
3.2
作者：
Harb, Amal;Awad, Dalal;Samarah, Nezar
通讯作者：
Samarah, Nezar