Characterization of intestinal invasion by Salmonella typhimurium and Salmonella dublin and effect of a mutation in the invH gene
Characterization of intestinal invasion by Salmonella typhimurium and Salmonella dublin and effect of a mutation in the invH gene
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鼠伤寒沙门氏菌和都柏林沙门氏菌肠道侵袭的特征以及 invH 基因突变的影响
DOI：
10.1128/iai.63.7.2743-2754.1995
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发表时间：
1995
影响因子：
3.1
通讯作者：
T. Wallis
中科院分区：
文献类型：
--
作者：
P. Watson;S. Paulin;A. Bland;P. W. Jones;T. Wallis
在 MDCK 和 Int 407 培养细胞测定以及牛回肠环中测定了鼠伤寒沙门氏菌和都柏林沙门氏菌的两种牛分离株以及鼠伤寒沙门氏菌 invH 突变体的相对侵袭性水平。研究发现，都柏林沙门氏菌对培养细胞的侵袭性明显低于鼠伤寒沙门氏菌，但在牛肠中未观察到这种差异。 invH突变体在培养细胞和牛肠中表现出显着的侵袭减少。通过荧光显微镜、扫描和透射电子显微镜证实了菌株的侵袭表型。野生型菌株在肠绒毛固有层中观察到，而相反，invH 突变体通常与肠上皮细胞层相关。牛回肠的损伤程度与入侵的程度有关。接种环后3小时，无论有或没有派尔氏斑，从牛回肠回收的鼠伤寒沙门氏菌或都柏林沙门氏菌的量没有差异。
The relative levels of invasiveness of two bovine isolates each of Salmonella typhimurium and Salmonella dublin and of invH mutants of S. typhimurium were determined in MDCK and Int 407 cultured-cell assays and in bovine ileal loops. S. dublin was found to be significantly less invasive in cultured cells than S. typhimurium, but this difference was not observed in bovine intestines. The invH mutants exhibited a significant reduction in invasion in both cultured cells and bovine intestines. The invasive phenotypes of the strains were confirmed by fluorescent microscopy and scanning and transmission electron microscopy. The wild-type strains were observed in the laminae propriae of the intestinal villi, while in contrast the invH mutants were generally associated with the enterocyte layer. The degree of damage in the bovine ileum was related to the magnitude of the invasion. There was no difference in the amount of S. typhimurium or S. dublin recovered from the bovine ileum either with or without Peyer's patches 3 h after inoculation of the loop.
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DOI：
10.1073/pnas.89.13.5976
发表时间：
1992-07-01
影响因子：
11.1
作者：
GINOCCHIO, C;PACE, J;GALAN, JE
通讯作者：
GALAN, JE
细菌与非吞噬细胞的相互作用。
影响因子：
7
作者：
Galán,JE
通讯作者：
Galán,JE
沙门氏菌对培养上皮细胞的粘附和侵袭相关基因的克隆和分子表征。
影响因子：
3.6
作者：
Altmeyer,RM;McNern,JK;Bossio,JC;Rosenshine,I;Finlay,BB;Galán,JE
通讯作者：
Galán,JE
微生物与肠道 M 细胞的相互作用：粘膜侵袭和分泌性免疫的诱导。
DOI：
--
发表时间：
1993
期刊：
Infectious agents and disease
影响因子：
--
作者：
Giannasca,PJ;Neutra,MR
通讯作者：
Neutra,MR
DOI：
10.1073/pnas.86.16.6383
发表时间：
1989-08-01
影响因子：
11.1
作者：
GALAN, JE;CURTISS, R
通讯作者：
CURTISS, R