A methodology for the production of microfabricated electrospun membranes for the creation of new skin regeneration models.
A methodology for the production of microfabricated electrospun membranes for the creation of new skin regeneration models.
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DOI：
10.1177/2041731418799851
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发表时间：
2018-01
影响因子：
8.2
通讯作者：
MacNeil S
中科院分区：
文献类型：
--
作者：
Asencio IO;Mittar S;Sherborne C;Raza A;Claeyssens F;MacNeil S
表皮的持续更新被认为与居住在受间充质成纤维细胞存在直接影响的物理保护微环境（微脊）中的表皮干细胞群体的存在有关。目前的体外皮肤模型确实承认了间质成纤维细胞在皮肤重组中的影响，但对微环境对表皮更新的影响的研究仍然是一个值得探索的丰富课题。我们建议有必要开发新的体外模型来研究上皮干细胞的行为，然后将这些模型转化为用于皮肤重建的新型无细胞生物材料装置的设计。在这项研究中，我们旨在开发包含假网状脊结构的新原型表皮样层，以研究地形线索对上皮细胞行为的影响。这些模型是用一系列三维静电纺丝微支架设计的。这是通过利用聚乙二醇二丙烯酸酯来生产可重复使用的模板来实现的，聚（3-羟基丁酸酯-co-3-羟基戊酸酯）在模板上进行静电纺丝。最初的研究研究了在使用普通支架（不存在复杂地形）的模型上培养的角质形成细胞与在含有微特征的支架上培养的角质形成细胞的行为。
The continual renewal of the epidermis is thought to be related to the presence of populations of epidermal stem cells residing in physically protected microenvironments (rete ridges) directly influenced by the presence of mesenchymal fibroblasts. Current skin in vitro models do acknowledge the influence of stromal fibroblasts in skin reorganisation but the study of the effect of the rete ridge-microenvironment on epidermal renewal still remains a rich topic for exploration. We suggest there is a need for the development of new in vitro models in which to study epithelial stem cell behaviour prior to translating these models into the design of new cell-free biomaterial devices for skin reconstruction. In this study, we aimed to develop new prototype epidermal-like layers containing pseudo-rete ridge structures for studying the effect of topographical cues on epithelial cell behaviour. The models were designed using a range of three-dimensional electrospun microfabricated scaffolds. This was achieved via the utilisation of polyethylene glycol diacrylate to produce a reusable template over which poly(3-hydrroxybutyrate-co-3-hydroxyvalerate) was electrospun. Initial investigations studied the behaviour of keratinocytes cultured on models using plain scaffolds (without the presence of intricate topography) versus keratinocytes cultured on scaffolds containing microfeatures.
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影响因子：
82.9
作者：
通讯作者：
--
影响因子：
--
作者：
Dhawan U;Pan HA;Shie MJ;Chu YH;Huang GS;Chen PC;Chen WL
通讯作者：
Chen WL
影响因子：
21.1
作者：
Quan C;Cho MK;Shao Y;Mianecki LE;Liao E;Perry D;Quan T
通讯作者：
Quan T
影响因子：
9.7
作者：
Vaquette, Cedryck;Cooper-White, Justin John
通讯作者：
Cooper-White, Justin John
影响因子：
5.3
作者：
Langton, Abigail K.;Halai, Poonam;Watson, Rachel E. B.
通讯作者：
Watson, Rachel E. B.