Tracking dynamic microvascular changes during healing after complete biopsy punch on the mouse pinna using optical microangiography.
Tracking dynamic microvascular changes during healing after complete biopsy punch on the mouse pinna using optical microangiography.
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DOI：
10.1371/journal.pone.0057976
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wang RK
中科院分区：
文献类型：
--
作者：
Jung Y;Dziennis S;Zhi Z;Reif R;Zheng Y;Wang RK
光学微血管造影术（OMAG）和多普勒光学微血管造影术（DOMAG）是两种能够确定组织微结构内容、微血管造影以及血流速度和方向的非侵入性技术。这些技术被用来可视化的急性和慢性微血管和组织反应后，在体内损伤。在小鼠耳廓上使用0.5 mm活检穿孔器诱导组织伤口。对急性（<30分钟）伤口反应的微血管造影、血流速度和方向的变化进行定量，并对慢性伤口反应（30分钟-60天）的组织结构和微血管造影的变化进行测定。最初的伤口引发招聘的外周毛细血管，以及在3分钟内的主要动脉和静脉血流的重定向。复杂的血管网络和新血管的形成进行了量化的慢性反应过程中使用分形维数。伤口闭合率最高的时间是第8天至第22天。在此期间，血管迂曲度增加，提示血管生成。综上所述，这些数据表明，OMAG有能力跟踪伤口愈合过程中血流、微血管造影和结构的急性和慢性变化。OMAG的使用具有很大的潜力，以提高我们对血管和组织对损伤的反应的理解，以开发更有效的治疗方法。
Optical microangiography (OMAG) and Doppler optical microangiography (DOMAG) are two non-invasive techniques capable of determining the tissue microstructural content, microvasculature angiography, and blood flow velocity and direction. These techniques were used to visualize the acute and chronic microvascular and tissue responses upon an injury in vivo. A tissue wound was induced using a 0.5 mm biopsy punch on a mouse pinna. The changes in the microangiography, blood flow velocity and direction were quantified for the acute (<30 min) wound response and the changes in the tissue structure and microangiography were determined for the chronic wound response (30 min–60 days). The initial wound triggered recruitment of peripheral capillaries, as well as redirection of main arterial and venous blood flow within 3 min. The complex vascular networks and new vessel formation were quantified during the chronic response using fractal dimension. The highest rate of wound closure occurred between days 8 and 22. The vessel tortuosity increased during this time suggesting angiogenesis. Taken together, these data signify that OMAG has the capability to track acute and chronic changes in blood flow, microangiography and structure during wound healing. The use of OMAG has great potential to improve our understanding of vascular and tissue responses to injury in order to develop more effective therapeutics.
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影响因子：
3.8
作者：
An L;Qin J;Wang RK
通讯作者：
Wang RK
影响因子：
7.6
作者：
Reif R;Qin J;An L;Zhi Z;Dziennis S;Wang R
通讯作者：
Wang R
影响因子：
2.4
作者：
Qin, Jia;Jiang, Jingying;An, Lin;Gareau, Daniel;Wang, Ruikang K.
通讯作者：
Wang, Ruikang K.
DOI：
10.1016/s0190-9622(99)70112-x
发表时间：
1999-09-01
影响因子：
13.8
作者：
Rapp, SR;Feldman, SR;Reboussin, DM
通讯作者：
Reboussin, DM
影响因子：
--
作者：
Lockwood, GR;Turnbull, DH;Foster, FS
通讯作者：
Foster, FS