Differential expression of MUC genes in endometrial and cervical tissues and tumors.
Differential expression of MUC genes in endometrial and cervical tissues and tumors.
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DOI：
10.1186/1471-2407-5-124
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发表时间：
2005-09-27
期刊：
影响因子：
3.8
通讯作者：
Sachdev, GP
中科院分区：
文献类型：
--
作者：
Hebbar, V;Damera, G;Sachdev, GP
粘蛋白糖蛋白是粘液的主要成分，被认为是一类重要的肿瘤相关抗原。本研究的目的是研究人类MUC基因(MUC1、MUC2、MUC5B、MUC5AC和MUC8)在人子宫内膜和宫颈组织中的表达，并对其在正常组织和癌组织中的表达进行比较和定量。用狭缝印迹技术研究MUC基因的表达和定量。在所研究的5个粘蛋白基因中，MUC1、MUC5B和MUC8在正常和癌组织中的表达水平较高，而MUC2和MUC5AC的表达水平较低。经统计学处理，子宫内膜腺癌组织中MUC1、MUC5B和MUC8的表达水平均高于正常子宫内膜组织。相反，与正常宫颈组织相比，宫颈肿瘤组织中只有MUC1表达增加，而MUC5B和MUC8的表达没有明显变化。子宫内膜肿瘤组织中MUC1、MUC5B和MUC8的表达均高于正常子宫内膜组织。在宫颈肿瘤中，只有MUC1似乎增加。所有研究的组织均显示MUC8mRNA的高表达和一致性。在所有研究的子宫内膜和宫颈组织中都观察到低水平的MUC2和MUC5AC。
Mucin glycoprotein's are major components of mucus and are considered an important class of tumor associated antigens. The objective of this study was to investigate the expression of human MUC genes (MUC1, MUC2, MUC5B, MUC5AC and MUC8) in human endometrium and cervix, and to compare and quantitate the expression of MUC genes in normal and cancerous tissues. Slot blot techniques were used to study the MUC gene expression and quantitation. Of the five-mucin genes studied, MUC1, MUC5B and MUC8 showed high expression levels in the normal and cancerous endometrial and cervical tissues, MUC2 and MUC5AC showed considerably lower expression. Statistically, higher levels of MUC1, MUC5B and MUC8 were observed in endometrial adenocarcinomas compared to normal tissues. In contrast, only MUC1 levels increased with no significant changes in expression of MUC5B and MUC8 in cervical tumors over normal cervical tissues. Endometrial tumors showed increased expression of MUC1, MUC5B and MUC8 over normal tissues. Only MUC1 appears to be increase, in cervical tumors. All the studied tissues showed high and consistent expression of MUC8 mRNA. Low to neglible levels of MUC2 and MUC5AC were observed in all studied endometrial and cervical tissues.
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影响因子：
6.1
作者：
AUDIE, JP;TETAERT, D;BOERSMA, A
通讯作者：
BOERSMA, A
影响因子：
--
作者：
LAYTON, GT;DEVINE, PL;MCKENZIE, IFC
通讯作者：
MCKENZIE, IFC
影响因子：
--
作者：
Nguyen, PL;Niehans, GA;Ho, SB
通讯作者：
Ho, SB
影响因子：
3.6
作者：
Gipson, IK;Spurr-Michaud, S;Hill, JA
通讯作者：
Hill, JA
DOI：
10.1165/ajrcmb/7.6.557
发表时间：
1992-12-01
影响因子：
6.4
作者：
GUM, JR
通讯作者：
GUM, JR