Identification and characterization of subpopulations of Pax3 and Pax7 expressing cells in developing chick somites and limb buds.
Identification and characterization of subpopulations of Pax3 and Pax7 expressing cells in developing chick somites and limb buds.
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DOI：
10.1002/dvdy.21585
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发表时间：
2008-07
影响因子：
2.5
通讯作者：
Burrus, Laura W.
中科院分区：
文献类型：
--
作者：
Galli, Lisa M.;Knight, Sara R.;Barnes, Tiffany L.;Doak, Allison K.;Kadzik, Rachel S.;Burrus, Laura W.
Pax3和Pax7是密切相关的成对盒式家族转录因子，在胚胎和成人肌肉发生中发挥重要作用。以往描述Pax3和Pax7转录本表达的报道揭示了Pax3和Pax7转录本在许多重叠区域中的表达。在这篇手稿中，我们通过检测Pax3和Pax7在发育中的鸡体节和四肢细胞分辨率的蛋白质表达谱来扩展这些研究。我们的研究表明，在躯体和发育肢体中存在不同的细胞亚群，这些细胞亚群由Pax3和Pax7的相对表达水平定义。我们还发现Pax3和Pax7相互负向调节彼此在皮肌节中的表达，从而为维持皮肌节中观察到的表达模式提供了一种可能的机制。皮肌节和肌节中Pax3和/或Pax7阳性细胞在增殖和分化方面的进一步特征揭示了具有不同特性的细胞亚群。
Pax3 and Pax7 are closely related paired-boxed family transcription factors that are known to play important roles in embryonic and adult myogenesis. Previous reports describing the expression of Pax3 and Pax7 transcripts reveal expression in many overlapping domains. In this manuscript, we extend these studies by examining the protein expression profiles for Pax3 and Pax7 in developing chick somites and limbs with cellular resolution. Our studies show the existence of distinct subpopulations of cells in the somite and developing limb that are defined by the relative expression levels of Pax3 and Pax7. We also show that Pax3 and Pax7 negatively regulate each other's expression in the dermomyotome, thus providing a possible mechanism for the maintenance of observed expression patterns in the dermomyotome. Further characterization of Pax3 and/or Pax7 positive cells in the dermomyotome and myotome with respect to proliferation and differentiation reveals subpopulations of cells with distinct properties.
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DOI：
10.1083/jcb.200508001
发表时间：
2006-01-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kuang S;Chargé SB;Seale P;Huh M;Rudnicki MA
通讯作者：
Rudnicki MA
影响因子：
2.7
作者：
Fan, CM;Lee, CS;TessierLavigne, M
通讯作者：
TessierLavigne, M
DOI：
10.1016/j.bbrc.2006.08.064
发表时间：
2006-10-20
影响因子：
3.1
作者：
Hsieh, Mei-Ju;Yao, Ya-Li;Yang, Wen-Ming
通讯作者：
Yang, Wen-Ming
影响因子：
2.6
作者：
JOSTES, B;WALTHER, C;GRUSS, P
通讯作者：
GRUSS, P
影响因子：
10.5
作者：
Kassar-Duchossoy, L;Giacone, E;Tajbakhsh, S
通讯作者：
Tajbakhsh, S