Early time-dependent dynamic changes of TBET and GATA3 mRNA expressions in patients with acute coronary syndrome.
Early time-dependent dynamic changes of TBET and GATA3 mRNA expressions in patients with acute coronary syndrome.
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DOI：
10.1155/2013/139895
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Yu CM
中科院分区：
文献类型：
--
作者：
Rainer TH;Graham CA;Chan RW;Chan CP;Tan PC;Yip GW;Yu CM
背景T细胞表达的T-box（TBET）和鸟嘌呤腺嘌呤胸腺嘧啶腺嘌呤序列结合蛋白3（GATA 3）在Th 1和Th 2亚群分化中起重要作用，从而促进急性冠状动脉综合征（ACS）的进展。Objective.本研究旨在探讨TBET/GATA 3 mRNA比值在ACS中的时相变化。方法.招募了33例在24小时内出现症状的疑似ACS患者。到达急诊科后每小时采集一次血样，直至第6小时。通过实时RT-qPCR定量TBET和GATA 3的mRNA表达。结果急性心肌梗死（AMI）患者TBET/GATA 3 mRNA比值显著升高，呈双相M型释放动力学。发病后2 h，比值升高，10 h降至最低水平，14 h升至第二高峰。在任何干预前采集血样的AMI患者中观察到类似的双相M形曲线。结论. TBET/GATA 3 mRNA比值在AMI患者症状发作后的前20小时内大部分时间内升高。双相M形释放动力学更可能反映病理生理学变化，而不是治疗效果。
Background. T-box expressed in T cells (TBET) and guanine adenine thymine adenine sequence-binding protein 3 (GATA3) play important roles in the differentiation of Th1 and Th2 subsets, which contributes to the progression of acute coronary syndrome (ACS). Objective. This study aimed to investigate the temporal change of TBET/GATA3 mRNA ratio in ACS. Methods. Thirty-three patients suspected of ACS with symptom onset within 24 hours were recruited. Blood samples were taken after arrival at the emergency department and at hourly intervals until the 6th hour. The mRNA expressions of TBET and GATA3 were quantified by a real-time RT-qPCR. Results. The TBET/GATA3 mRNA ratio was elevated dramatically in patients with acute myocardial infarction (AMI) and exhibited biphasic M-shaped release kinetics with two distinct peaks. The ratio was elevated 2 hours after symptom onset, dropped to the lowest level at 10 hours, and rose to the second peak at 14 hours. A similar biphasic M-shaped curve was observed in AMI patients with blood samples taken prior to any intervention. Conclusions. The TBET/GATA3 mRNA ratio was elevated in AMI patients throughout most of the first 20 hours after symptom onset. The biphasic M-shaped release kinetics was more likely to reflect pathophysiological changes rather than treatment effects.
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