Probing heavy metal binding to phycobiliproteins.
Probing heavy metal binding to phycobiliproteins.
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探测重金属结合与植物脂蛋白的结合。
DOI：
10.1111/febs.16396
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发表时间：
2022-08
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影响因子：
--
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中科院分区：
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--
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蓝藻，也被称为蓝藻，含有一些已知的最有效的捕光复合体。这些大型的、五颜六色的复合体由藻胆蛋白组成，这些蛋白在化妆品、食品、营养食品和制药行业中非常有价值。此外，藻胆蛋白的彩色和荧光特性可以被金属离子调节，使其作为重金属传感器和重金属清除剂具有很高的吸引力。虽然金属离子对藻胆蛋白具有整体猝灭能力是已知的，但重金属与藻胆蛋白结合的机制还不完全清楚，限制了其广泛的定量应用。在这里，我们使用高分辨率自然质谱学显示，藻胆蛋白复合体以不同的方式结合金属离子。通过监测结合平衡和金属结合化学计量学，我们发现特别是铜和银对藻胆蛋白结构有强烈但不同的影响，铜和银都调节着整个络合物的性质。综上所述，这些数据揭示了金属离子调节藻胆蛋白特性的机制，这可以作为未来设计金属相关藻胆蛋白应用的基础。天然质谱仪显示金属离子与来自蓝藻的彩色荧光藻胆蛋白的不同结合。这项研究的结果对藻胆蛋白在生物传感器中的使用以及在预测蓝藻对环境中重金属污染的反应方面具有广泛的影响。
Blue‐green algae, also known as cyanobacteria, contain some of the most efficient light‐harvesting complexes known. These large, colourful complexes consist of phycobiliproteins which are extremely valuable in the cosmetics, food, nutraceutical and pharmaceutical industries. Additionally, the colourful and fluorescent properties of phycobiliproteins can be modulated by metal ions, making them highly attractive as heavy metal sensors and heavy metal scavengers. Although the overall quenching ability metal ions have on phycobiliproteins is known, the mechanism of heavy metal binding to phycobiliproteins is not fully understood, limiting their widespread quantitative applications. Here, we show using high‐resolution native mass spectrometry that phycobiliprotein complexes bind metal ions in different manners. Through monitoring the binding equilibria and metal‐binding stoichiometry, we show in particular copper and silver to have drastic, yet different effects on phycobiliprotein structure, both copper and silver modulate the overall complex properties. Together, the data reveals the mechanisms by which metal ions can modulate phycobiliprotein properties which can be used as a basis for the future design of metal‐related phycobiliprotein applications. Native mass spectrometry reveals differential binding of metal ions to the colourful fluorescent phycobiliproteins from cyanobacteria. The results presented in this study have a broad impact on the use of phycobiliproteins in biosensors and in predicting the response of cyanobacteria to heavy‐metal contaminants within the environment.
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