Metabolic response of the sea cucumber Apostichopus japonicus during the estivation-arousal cycles
Metabolic response of the sea cucumber Apostichopus japonicus during the estivation-arousal cycles
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海参夏眠-觉醒周期的代谢反应
DOI：
10.3389/fmars.2022.980221
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发表时间：
2022-08
影响因子：
3.7
通讯作者：
Muyan Chen
中科院分区：
文献类型：
--
作者：
Ye Zhao;Haona Wang;Han Wang;Yongrui Pi;Muyan Chen
刺激是一种广泛的生存策略，用于应对不利的环境条件，如高温，低氧和缺乏水或食物，这已经在多个脊椎动物和无脊椎动物物种中报道。仿刺参是研究海洋无脊椎动物夏眠适应机制的理想模式生物。本研究采用超高效液相色谱-电喷雾飞行时间质谱联用技术（UPLC-ESI-Q-TOF/MS）研究了A.在一年的夏眠-觉醒周期中：无夏眠（NA），深夏眠（DA）和夏眠觉醒（AA）。共鉴定出424种代谢产物，并在DA与NA、AA与DA和AA与NA的比较中进一步筛选出243、238和37种显著差异代谢产物（DM）。具体而言，参与糖酵解和三羧酸循环的代谢产物的水平显著降低，而在A中发现更高量的长链脂肪酸、磷脂和游离氨基酸。这表明海参可能通过抑制碳水化合物代谢、促进脂肪和氨基酸代谢来重组ATP生成的代谢优先级。有趣的是，进入戊糖磷酸途径的相对碳流量增加和各种非酶抗氧化剂分子（例如，生育三烯酚、叶酸、儿茶素、染料木黄酮和白藜芦醇），这表明活性氧防御系统的增强可能以能量有效的方式促进低代谢状态下的长期生存能力。因此，本研究为海洋无脊椎动物在代谢水平上对夏眠的适应机制提供了新的见解。
Estivation is a widespread survival strategy for dealing with adverse environmental conditions such as high temperature, low oxygen and lack of water or food, which has been reported across multiple vertebrate and invertebrate species. The sea cucumber Apostichopus japonicus is an excellent model organism to investigate the adaptive mechanism of estivation in marine invertebrates. In this study, a metabolomics approach based on ultraperformance liquid chromatography coupled with electrospray ionization time-of-flight mass spectrometry (UPLC-ESI-Q-TOF/MS) was performed to reveal the metabolic response of intestines from adult A. japonicus over the annual estivation-arousal cycle: nonestivation (NA), deep-estivation (DA) and arousal from estivation (AA). A total of 424 metabolites were identified, and among them, 243, 238 and 37 significant differentially metabolites (DMs) were further screened in the comparisons of DA vs. NA, AA vs. DA, and AA vs. NA. Specifically, the levels of metabolites involved in glycolysis and the tricarboxylic acid cycle were significantly decreased, while higher amounts of long-chain fatty acids, phospholipids and free amino acids were found in A. japonicus during estivation, implying that sea cucumbers might reorganize metabolic priorities for ATP production by depressing carbohydrate metabolism and promoting lipid and amino acid catabolism. Interestingly, elevated relative carbon flow entry into the pentose phosphate pathway and accumulation of various nonenzymatic antioxidant molecules (e.g., tocotrienols, folic acid, catechin, genistein and resveratrol) were observed in estivating sea cucumbers, which suggested that enhancement of the reactive oxygen species defense system might promote long-term viability in the hypometabolic state in an energy-efficient manner. Thus, this research provides new insights into the adaptation mechanisms of marine invertebrates to estivation at the metabolic level.
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影响因子：
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作者：
Simioni C;Zauli G;Martelli AM;Vitale M;Sacchetti G;Gonelli A;Neri LM
通讯作者：
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影响因子：
3.7
作者：
Chen M;Zhang X;Liu J;Storey KB
通讯作者：
Storey KB
影响因子：
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作者：
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