Chemical epigenetics alters the secondary metabolite composition of guttate excreted by an atlantic-forest-soil-derived Penicillium citreonigrum.
Chemical epigenetics alters the secondary metabolite composition of guttate excreted by an atlantic-forest-soil-derived Penicillium citreonigrum.
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DOI：
10.1021/np100142h
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发表时间：
2010-05-28
影响因子：
5.1
通讯作者：
Cichewicz RH
中科院分区：
文献类型：
--
作者：
Wang X;Sena Filho JG;Hoover AR;King JB;Ellis TK;Powell DR;Cichewicz RH
化学表观遗传操作的柑橘黑青霉菌导致其滴液中的次级代谢产物谱的深刻变化。虽然对照培养物的滴液显示出相对简单的次级代谢产物组合，但从50 μM 5-氮杂胞苷（DNA甲基转移酶抑制剂）处理的培养物中收集的滴液高度富集代表至少三个不同生物合成家族的化合物。从该真菌中获得的代谢产物包括六种azaphilones（小球藻素（1）、小球藻胺（6）、赭曲霉酮（2）、脱氯异色酚酮III（3）、脱氯异色酚酮IV（4）和6-（（3E，5E）-5，7-二甲基-2-亚甲基壬-3，5-二烯基）-2，4-二羟基-3-甲基苯甲醛（5））、pencolide（7），和两个新的部分萜类化合物（atlantinone A和B（分别为9和10））。虽然在对照和5-氮杂胞苷处理的培养物的渗出物中检测到pencolide，但所有其他天然产物仅在表观遗传修饰的真菌的滴液中发现。在纸片扩散测定法中测试来自柠檬黑青霉点滴液的所有代谢物的抗微生物活性。scleroclavin和scleroclavamine都引起表皮葡萄球菌生长的适度抑制;然而，只有scleroclavamine对念珠菌属菌株的一组有活性。
Chemical epigenetic manipulation of Penicillium citreonigrum led to profound changes in the secondary metabolite profile of its guttate. While guttate from control cultures exhibited a relatively simple assemblage of secondary metabolites, the guttate collected from cultures treated with 50 μM 5-azacytidine (a DNA methyltransferase inhibitor) were highly enriched in compounds representing at least three distinct biosynthetic families. The metabolites obtained from the fungus included six azaphilones (sclerotiorin (1), sclerotioramine (6), ochrephilone (2), dechloroisochromophilone III (3), dechloroisochromophilone IV (4), and 6-((3E,5E)-5,7-dimethyl-2-methylenenona-3,5-dienyl)-2,4-dihydroxy-3-methylbenzaldehyde (5)), pencolide (7), and two new meroterpenes (atlantinones A and B (9 and 10, respectively)). While pencolide was detected in the exudates of both control and 5-azacytidine-treated cultures, all of the other natural products were found exclusively in the guttates of the epigenetically modified fungus. All of the metabolites from the P. citreonigrum guttate were tested for antimicrobial activity in a disk diffusion assay. Both sclerotiorin and sclerotioramine caused modest inhibition of Staphylococcus epidermidis growth; however, only sclerotioramine was active against a panel of Candida strains.
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影响因子：
1.8
作者：
Shiomi, K;Uchida, R;Omura, S
通讯作者：
Omura, S
影响因子：
5.5
作者：
Gareis, Manfred;Gareis, Eva-Maria
通讯作者：
Gareis, Eva-Maria
影响因子：
2.8
作者：
Hutwimmer, Stefan;Wang, Hui;Burgstaller, Wolfgang
通讯作者：
Burgstaller, Wolfgang
DOI：
10.1139/b77-045
发表时间：
1977-01-01
期刊：
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
影响因子：
--
作者：
MCPHEE, WJ;COLOTELO, N
通讯作者：
COLOTELO, N
影响因子：
2.1
作者：
Quang, DN;Hashimoto, T;Asakawa, Y
通讯作者：
Asakawa, Y