Uracil content of 16S rRNA of thermophilic and psychrophilic prokaryotes correlates inversely with their optimal growth temperatures.
Uracil content of 16S rRNA of thermophilic and psychrophilic prokaryotes correlates inversely with their optimal growth temperatures.
复制标题
嗜热和精神病原核生物的16S rRNA的尿嘧啶含量与它们的最佳生长温度相反。
DOI：
10.1093/nar/gki714
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发表时间：
2005
影响因子：
14.9
通讯作者：
dos Santos, VAPM
中科院分区：
文献类型：
--
作者：
Khachane, AN;Timmis, KN;dos Santos, VAPM
我们在这里报告的发现一个非常显着的负相关的尿嘧啶含量的16S rRNA和培养的嗜热和嗜冷原核生物的最佳生长温度（Topt）。这种相关性与其他核苷酸含量与Topt之间的弱相关性显著不同。16S rRNA二级结构区的分析显示，A：U碱基对含量的下降与Topt的增加同步，这比错配碱基对的增加要陡得多，错配碱基对的稳定性较差。这些结果表明，16S rRNA序列的嗜热菌和嗜冷菌是在一个强大的热适应压力下，结构功能的限制在决定其16S rRNA核苷酸组成起着至关重要的作用。尿嘧啶含量和Topt之间的衍生关系被用来开发一种算法来预测Topt值的未培养的原核生物缺乏培养的近亲，属于门主要含有嗜热菌。该算法可用于指导迄今未培养的微生物的培养条件的设计。
We report here the finding of a highly significant inverse correlation of the uracil content of 16S rRNA and the optimum growth temperature (Topt) of cultured thermophilic and psychrophilic prokaryotes. This correlation was significantly different from the weaker correlations between the contents of other nucleotides and Topt. Analysis of the 16S rRNA secondary structure regions revealed a fall in the A:U base-pair content in step with the increase in Topt that was much steeper than that of mismatched base-pairs, which are thermodynamically less stable. These findings indicate that the 16S rRNA sequences of thermophiles and psychrophiles are under a strong thermo-adaptive pressure, and that structure–function constraints play a crucial role in determining their 16S rRNA nucleotide composition. The derived relationship between uracil content and Topt was used to develop an algorithm to predict the Topt values of uncultured prokaryotes lacking cultured close relatives and belonging to the phyla predominantly containing thermophiles. This algorithm may be useful in guiding the design of cultivation conditions for hitherto uncultured microbes.
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影响因子：
3.2
作者：
Martins, LO;Carreto, LS;Santos, H
通讯作者：
Santos, H
影响因子：
14.9
作者：
Kreil, DP;Ouzounis, CA
通讯作者：
Ouzounis, CA
影响因子：
3
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通讯作者：
Gutell RR
影响因子：
4.4
作者：
Purdy, KJ;Nedwell, DB;Embley, TM
通讯作者：
Embley, TM
影响因子：
3.9
作者：
Rahman, TJ;Marchant, R;Banat, IM
通讯作者：
Banat, IM