Quantification of adiposity in small rodents using micro-CT.
Quantification of adiposity in small rodents using micro-CT.
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DOI：
10.1016/j.ymeth.2009.05.017
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发表时间：
2010-01
期刊：
影响因子：
4.8
通讯作者：
Rubin, C. T.
中科院分区：
文献类型：
--
作者：
Judex, S.;Luu, Y. K.;Ozcivici, E.;Adler, B.;Lublinsky, S.;Rubin, C. T.
关键词：
活体啮齿动物的非侵入性三维成像是一种强大的研究工具，对于许多生物医学领域的进步至关重要。对于脂肪发育、肥胖或糖尿病的研究，精确和精确的体内肥胖量化技术至关重要。由于全身脂肪量不能准确预测与代谢综合征相关的健康风险，因此影像学方法应该能够将全身肥胖分为皮下和内脏肥胖。微型计算机断层扫描（micro-CT）根据材料的物理密度获取高分辨率图像，可以很容易地区分皮下脂肪和内脏脂肪。在这里，描述了一种基于微CT的方法来对活啮齿动物的肥胖进行成像。一种自动化和验证的算法来量化计算机断层扫描的离散脂肪沉积物的体积是可用的。数据表明，扫描腹部提供了足够的信息来估计全身脂肪。micro-CT确定的脂肪体积和脂肪垫重量之间的高度相关性表明，micro-CT可以准确地监测肥胖的部位特异性变化。总之，体内micro-CT是一种非侵入性的、高度定量的成像方式，具有更高的分辨率和选择性，但可能比许多其他方法更低的吞吐量，以精确地确定活啮齿动物中的总脂肪体积和局部脂肪体积以及脂肪浸润。
Non-invasive three-dimensional imaging of live rodents is a powerful research tool that has become critical for advances in many biomedical fields. For investigations into adipose development, obesity, or diabetes, accurate and precise techniques that quantify adiposity in vivo are critical. Because total body fat mass does not accurately predict health risks associated with the metabolic syndrome, imaging modalities should be able to stratify total adiposity into subcutaneous and visceral adiposity. Micro-computed tomography (micro-CT) acquires high-resolution images based on the physical density of the material and can readily discriminate between subcutaneous and visceral fat. Here, a micro-CT based method to image the adiposity of live rodents is described. An automated and validated algorithm to quantify the volume of discrete fat deposits from the computed tomography is available. Data indicate that scanning the abdomen provides sufficient information to estimate total body fat. Very high correlations between micro-CT determined adipose volumes and the weight of explanted fat pads demonstrate that micro-CT can accurately monitor site-specific changes in adiposity. Taken together, in vivo micro-CT is a non-invasive, highly quantitative imaging modality with greater resolution and selectivity, but potentially lower through-put, than many other methods to precisely determine total and regional adipose volumes and fat infiltration in live rodents.
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