Antibodies to the L1 adhesion molecule inhibit Schwann cell ensheathment of neurons in vitro.
Antibodies to the L1 adhesion molecule inhibit Schwann cell ensheathment of neurons in vitro.
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DOI：
10.1083/jcb.109.6.3095
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发表时间：
1989-12
期刊：
影响因子：
--
通讯作者：
Schachner M
中科院分区：
文献类型：
--
作者：
Seilheimer B;Persohn E;Schachner M
为了研究神经粘附分子是否参与神经元诱导的许旺细胞分化，在抗体存在下维持纯背根神经节神经元和许旺细胞的共培养物以评估可能的干扰作用。研究了雪旺细胞分化的几个特征参数，如梭形向扁平的转变，即，更多的上皮样形态、与神经元细胞体的结合、神经突的鞘化、基底层和胶原原纤维的产生以及髓鞘相关糖蛋白（MAG）的表达。神经粘附分子L1抗体完全消融雪旺细胞分化的所有功能研究。N-CAM的抗体并没有减少雪旺细胞与神经突的关联，但取消了雪旺细胞突起成神经突束的交错，同时使其他研究参数不受影响。Fab片段的抗体J1，MAG和小鼠肝膜没有干扰任何这些参数的表现。所有抗体均未改变[3 H]胸苷掺入雪旺细胞的情况。
To investigate whether neural adhesion molecules are involved in neuron- induced Schwann cell differentiation, cocultures of pure dorsal root ganglion neurons, and Schwann cells were maintained in the presence of antibodies to evaluate possible perturbing effects. Several parameters characteristic of differentiating Schwann cells were studied, such as transition of spindle-shaped to flattened, i.e., more epithelioid morphology, association with neuronal cell bodies, ensheathment of neurites, production of basal lamina and collagen fibrils, and expression of the myelin associated glycoprotein (MAG). A complete ablation of Schwann cell differentiation in all features studied was seen with antibodies to the neural adhesion molecule L1. Antibodies to N-CAM did not reduce the association of Schwann cells with neurites but abolished the interdigitation of Schwann cell processes into neurite bundles, while leaving the other parameters studied unaffected. Fab fragments of antibodies to J1, MAG, and mouse liver membranes did not interfere with the manifestation of any of these parameters. None of the antibodies changed incorporation of [3H]thymidine into Schwann cells.
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影响因子：
7.8
作者：
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通讯作者：
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影响因子：
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6.2
作者：
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