Biomimetic poly(ethylene glycol)-based hydrogels as scaffolds for inducing endothelial adhesion and capillary-like network formation.
Biomimetic poly(ethylene glycol)-based hydrogels as scaffolds for inducing endothelial adhesion and capillary-like network formation.
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DOI：
10.1021/bm201596w
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发表时间：
2012-03-12
影响因子：
6.2
通讯作者：
Marchant, Roger E.
中科院分区：
文献类型：
--
作者：
Zhu, Junmin;He, Ping;Lin, Lin;Jones, Derek R.;Marchant, Roger E.
细胞外基质（ECM）是一种有吸引力的模型，用于设计具有理想环境的组织工程合成支架。在这里，我们报告的ECM模拟聚（乙二醇）（PEG）水凝胶的合成诱导内皮细胞（EC）粘附和毛细血管样网络的形成。将胶原酶敏感的GIA序列连接到聚乙二醇（PEGDA）链的中间，合成了一种I型胶原衍生肽--GPQGIAGQ（GIA）-PEGDA（GIA-PEGDA），然后将其与RGD封端的聚乙二醇单丙烯酸酯（RGD-PEGMA）共聚形成仿生水凝胶。该凝胶在体外的降解速率依赖于胶原酶的浓度，并支持人脐静脉内皮细胞（HUVECs）的粘附。当HUVECs接种在水凝胶表面时，将1% RGD-PEGDA掺入到GIA-PEGDA水凝胶中的仿生RGD/GIA-PEGDA水凝胶诱导毛细血管样组织，而RGD/PEGDA和GIA-PEGDA水凝胶没有。这些结果表明，细胞粘附和支架的生物降解性在毛细血管样网络的形成中起重要作用。
The extracellular matrix (ECM) is an attractive model for designing synthetic scaffolds with a desirable environment for tissue engineering. Here, we report on the synthesis of ECM-mimetic poly(ethylene glycol) (PEG) hydrogels for inducing endothelial cell (EC) adhesion and capillary-like network formation. A collagen type I-derived peptide, GPQGIAGQ (GIA)-containing PEGDA (GIA-PEGDA) was synthesized with the collagenase-sensitive GIA sequence attached in the middle of the PEGDA chain, which was then copolymerized with RGD capped-PEG monoacrylate (RGD-PEGMA) to form biomimetic hydrogels. The hydrogels degraded in vitro with the rate dependent on the concentration of collagenase, and also supported the adhesion of human umbilical vein ECs (HUVECs). Biomimetic RGD/GIA-PEGDA hydrogels with incorporation of 1% RGD-PEGDA into GIA-PEGDA hydrogels induced capillary-like organization when HUVECs were seeded on the hydrogel surface, while RGD/PEGDA and GIA-PEGDA hydrogels did not. These results indicate that both cell adhesion and biodegradability of scaffolds play important roles in the formation of capillary-like networks.
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影响因子：
20.1
作者：
Chen, JL;Zhang, ZG;Chopp, M
通讯作者：
Chopp, M
影响因子：
3.7
作者：
Gaebel R;Furlani D;Sorg H;Polchow B;Frank J;Bieback K;Wang W;Klopsch C;Ong LL;Li W;Ma N;Steinhoff G
通讯作者：
Steinhoff G
影响因子：
--
作者：
Elbjeirami, WM;West, JL
通讯作者：
West, JL
影响因子：
3.6
作者：
Bryant, SJ;Nuttelman, CR;Anseth, KS
通讯作者：
Anseth, KS
影响因子：
6.7
作者：
Atala, Anthony
通讯作者：
Atala, Anthony