Immunohistochemical evidence of Muc1 expression during rat embryonic development.
Immunohistochemical evidence of Muc1 expression during rat embryonic development.
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DOI：
10.4081/ejh.2010.e49
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发表时间：
2010-11-26
期刊：
影响因子：
--
通讯作者：
Croce MV
中科院分区：
文献类型：
--
作者：
Lacunza E;Ferretti V;Barbeito C;Segal-Eiras A;Croce MV
在胚胎发育过程中，对小鼠和人类胚胎的研究表明，Muc1/MUC1 的表达与上皮层和腺体形成的开始一致。因此，本研究旨在评估 Muc1 在大鼠发育不同阶段的时间和空间表达。在本报告中，纳入了 80 只动物：64 只来自怀孕雌性 (WKAH/Hok) 的妊娠第 13、14、15、16、17、18、19 和 20 天的大鼠胎儿，每个阶段 8 个胚胎。使用抗 MUC1 胞质尾多克隆抗体 (CT33) 进行标准免疫组织化学分析。当超过5%的细胞被染色时，认为反应呈阳性；反应模式为：L = 线性、膜，C = 细胞质，M = 混合；还记录了核染色。强度分为负（−）、低（+）、中（++）和强（+++）。第13天（13 D）在胃、肺和肾中观察到Muc1低强度表达； 14天时，小肠和胰腺也出现反应； 16 天，肝脏和食道，18 天，气管和唾液腺。在发育过程中，强度增加，而表达模式发生变化：在妊娠的第一天，它主要是线性和顶端的，而在进一步发育过程中，观察到细胞质表达的增加。气管、胃、肾和肺上皮是反应性最强的组织。在新生儿和成人的样本中，所有分析的组织都显示出相似的 Muc1 表达。本研究的结果评估 Muc1 在上皮大鼠胚胎发育中高度表达。
During embryonic development, studies on mouse and human embryos have established that Muc1/MUC1 expression coincides with the onset of epithelial sheet and glandular formation. This study aimed therefore at evaluating the temporal and spatial expression of Muc1 at different stages of rat development. In this report, 80 animals were included: 64 rat foetuses at 13, 14, 15, 16, 17, 18, 19 and 20 days of gestation from pregnant females (WKAH/Hok), 8 embryos each stage. Standard immunohistochemistry was performed using anti-MUC1 cytoplasmic tail polyclonal antibody (CT33). The reaction was considered positive when more than 5% of the cells were stained; reaction patterns were: L = linear, membrane, C = cytoplasmic and M = mixed; nuclear staining was also recorded. Intensity was graded as negative (−), low (+), moderate (++) and strong (+++). Muc1 expression was observed with a low intensity on 13th day (13 D) in the stomach, lung and kidney; at 14 d, small intestine and pancreas were also reactive; at 16 D, liver and esophagus and at 18 D, trachea and salivary glands. During the development, intensity increased while the pattern of expression changed: at the first days of gestation, it was predominantly linear and apical while during further development an increase in cytoplasmic expression was observed. Trachea, stomach, kidney and lung epithelia were the more reactive tissues. In specimens belonging to neonates and adults, all tissues analyzed showed similar Muc1 expression. The findings of this study assess that Muc1 is highly expressed in the epithelial rat embryonic development.
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DOI：
10.1016/s0006-291x(03)00662-4
发表时间：
2003-05-23
影响因子：
3.1
作者：
Fiegel, HC;Kluth, J;Kluth, D
通讯作者：
Kluth, D
影响因子：
3.2
作者：
Croce, MV;Isla-Larrain, MT;Segal-Eiras, A
通讯作者：
Segal-Eiras, A
影响因子：
2.4
作者：
Lacunza, Ezequiel;Bara, Jacques;Croce, Maria V.
通讯作者：
Croce, Maria V.
DOI：
10.1165/ajrcmb/8.5.546
发表时间：
1993-05-01
影响因子：
6.4
作者：
RANDELL, SH;SHIMIZU, T;NETTESHEIM, P
通讯作者：
NETTESHEIM, P
影响因子：
6.4
作者：
BURCHELL, J;WANG, D;TAYLORPAPADIMITRIOU, J
通讯作者：
TAYLORPAPADIMITRIOU, J