Small RNA-mediated silencing of phototropin suppresses the induction of photoprotection in the green alga Chlamydomonas reinhardtii.
Small RNA-mediated silencing of phototropin suppresses the induction of photoprotection in the green alga Chlamydomonas reinhardtii.
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DOI：
10.1073/pnas.2302185120
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发表时间：
2023-05-02
影响因子：
11.1
通讯作者：
Minagawa, Jun
中科院分区：
文献类型：
--
作者：
Yamasaki, Tomohito;Tokutsu, Ryutaro;Sawa, Haruhi;Razali, Nazifa Naziha;Hayashi, Momoka;Minagawa, Jun
小RNA(SRNAs)是重要的非编码RNA，作用于含有互补序列的mRNAs来控制基因表达，从而调节多细胞生物体中的各种生理现象。这种机制广泛存在于多细胞真核生物中，并被认为是在动植物的最后一个共同祖先--单细胞生物中建立的，但它在现存的单细胞真核生物中的作用尚未得到很好的研究。在本研究中，我们确定了sRNA调节单细胞绿藻Chlamydomonas reinhardtii中蓝光受体Phototroin的丰度，并参与了由蓝光信号驱动的光合作用系统对过量光的光保护机制的调节。小RNA(SRNAs)与ArgAerte蛋白形成复合物，并与具有互补序列的转录本结合，以抑制基因表达。SRNA介导的调控在多种真核生物中都是保守的，并参与各种生理功能的调控。RRNA存在于单细胞绿藻Chlamydomonas reinhardtii中，遗传分析表明其核心的sRNA的生物发生和作用机制与多细胞生物的sRNA和作用机制是保守的。然而，sRNAs在该生物体中的作用在很大程度上仍不清楚。在这里，我们报告衣藻sRNA有助于光保护的诱导。在这种藻类中，光保护是由捕光应激相关复合体3(LHCSR3)介导的，它的表达是由光信号通过蓝光受体趋光素(PHOT)诱导的。我们在这里证明，sRNA缺陷突变体显示出更多的PHOT丰度导致更高的LHCSR3表达。破坏两个预计与PHOT转录本结合的sRNA的前体也增加了PHOT的积累和LHCSR3的表达。蓝光对突变体LHCSR3的诱导作用明显增强，而红光对LHCSR3的诱导作用不明显，表明sRNAs通过调节PHOT的表达来调节光保护程度。我们的结果表明，sRNAs不仅参与光保护的调节，而且还参与PHOT信号调节的生物现象。
Small RNAs (sRNAs) are important noncoding RNAs that act on mRNAs harboring complementary sequences to control gene expression and thereby regulate various physiological phenomena in multicellular organisms. This mechanism is widely present in multicellular eukaryotes and is thought to have been established in the last common ancestor of animals and plants, which was a unicellular organism, but its role in extant unicellular eukaryotes has not been well studied. In this study, we determined that sRNAs regulate the abundance of the blue-light receptor phototropin in the unicellular green alga Chlamydomonas reinhardtii and are involved in the regulation of a photoprotection mechanism of the photosynthetic system against excess light, which is driven by blue-light signaling. Small RNAs (sRNAs) form complexes with Argonaute proteins and bind to transcripts with complementary sequences to repress gene expression. sRNA-mediated regulation is conserved in a diverse range of eukaryotes and is involved in the control of various physiological functions. sRNAs are present in the unicellular green alga Chlamydomonas reinhardtii, and genetic analyses revealed that the core sRNA biogenesis and action mechanisms are conserved with those of multicellular organisms. However, the roles of sRNAs in this organism remain largely unknown. Here, we report that Chlamydomonas sRNAs contribute to the induction of photoprotection. In this alga, photoprotection is mediated by LIGHT HARVESTING COMPLEX STRESS-RELATED 3 (LHCSR3), whose expression is induced by light signals through the blue-light receptor phototropin (PHOT). We demonstrate here that sRNA-defective mutants showed increased PHOT abundance leading to greater LHCSR3 expression. Disruption of the precursor for two sRNAs predicted to bind to the PHOT transcript also increased PHOT accumulation and LHCSR3 expression. The induction of LHCSR3 in the mutants was enhanced by light containing blue wavelengths, but not by red light, indicating that the sRNAs regulate the degree of photoprotection via regulation of PHOT expression. Our results suggest that sRNAs are involved not only in the regulation of photoprotection but also in biological phenomena regulated by PHOT signaling.
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影响因子：
18
作者：
Chung BY;Deery MJ;Groen AJ;Howard J;Baulcombe DC
通讯作者：
Baulcombe DC
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影响因子：
6.3
作者：
Lou S;Sun T;Li H;Hu Z
通讯作者：
Hu Z
影响因子：
7.2
作者：
Molnar, Attila;Bassett, Andrew;Baulcombe, David
通讯作者：
Baulcombe, David
影响因子：
3.7
作者：
Müller SY;Matthews NE;Valli AA;Baulcombe DC
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Baulcombe DC
影响因子：
11.6
作者：
Axtell, Michael J.;Meyers, Blake C.
通讯作者：
Meyers, Blake C.