Reduced Graphene Oxide and Gold Nanoparticles-Modified Electrochemical Aptasensor for Highly Sensitive Detection of Doxorubicin.
Reduced Graphene Oxide and Gold Nanoparticles-Modified Electrochemical Aptasensor for Highly Sensitive Detection of Doxorubicin.
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DOI：
10.3390/nano13071223
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发表时间：
2023-03-30
期刊：
影响因子：
--
通讯作者：
Cai X
中科院分区：
文献类型：
--
作者：
Kong F;Luo J;Jing L;Wang Y;Shen H;Yu R;Sun S;Xing Y;Ming T;Liu M;Jin H;Cai X
多柔比星（DOX）是临床上最重要的抗肿瘤抗生素，但其严重的心脏毒性和其他副作用限制了其临床应用。因此，在治疗期间监测DOX浓度对于提高疗效和减少不良反应至关重要。在这里，我们制作了一个敏感的电化学适配器检测DOX。该传感器使用金丝作为工作电极，并用还原氧化石墨烯（rGO）/金纳米颗粒（AuNPs）修饰以提高灵敏度。使用适体作为DOX的识别元件。适体的5′端用巯基修饰，从而固定到AuNPs上，3′端用亚甲蓝修饰，其用作电子介体。适体和DOX之间的组合将产生结合诱导的构象，其改变电子转移速率，产生与DOX浓度相关的电流变化。在0.3 μM ~ 6 μM浓度范围内，适体传感器的线性关系良好，检测限为0.1 μM。此外，将该适体传感器用于真实的样品和结果中的DOX检测，并显示出良好的回收率。该电化学适体传感器将为DOX的检测提供一个灵敏、快速、简便、可靠的新平台。
Doxorubicin (DOX) is the most clinically important antibiotic in cancer treatment, but its severe cardiotoxicity and other side effects limit its clinical use. Therefore, monitoring DOX concentrations during therapy is essential to improve efficacy and reduce adverse effects. Here, we fabricated a sensitive electrochemical aptasensor for DOX detection. The sensor used gold wire as the working electrode and was modified with reduced graphene oxide (rGO)/gold nanoparticles (AuNPs) to improve the sensitivity. An aptamer was used as the recognition element for the DOX. The 5′ end of the aptamer was modified with a thiol group, and thus immobilized to the AuNPs, and the 3′ end was modified with methylene blue, which acts as the electron mediator. The combination between the aptamer and DOX would produce a binding-induced conformation, which changes the electron transfer rate, yielding a current change that correlates with the concentration of DOX. The aptasensor exhibited good linearity in the DOX concentration range of 0.3 μM to 6 μM, with a detection limit of 0.1 μM. In addition, the aptasensor was used for DOX detection in real samples and results, and showed good recovery. The proposed electrochemical aptasensor will provide a sensitive, fast, simple, and reliable new platform for detecting DOX.
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DOI：
10.3390/bios8010007
发表时间：
2018-01-15
期刊：
Biosensors
影响因子：
--
作者：
Khan NI;Maddaus AG;Song E
通讯作者：
Song E
影响因子：
4.3
作者：
Bahner, Nicole;Reich, Peggy;Beckmann, Dieter
通讯作者：
Beckmann, Dieter
DOI：
10.1016/s0731-7085(99)00048-5
发表时间：
1999-06-01
影响因子：
3.4
作者：
Hassan, HNA;Barsoum, BN;Habib, IHI
通讯作者：
Habib, IHI
一种低温制备高度还原氧化石墨烯的方法
影响因子：
16.6
作者：
Feng, Hongbin;Cheng, Rui;Li, Jinghong
通讯作者：
Li, Jinghong
影响因子：
3.3
作者：
Ates, Ali Kemal;Er, Engin;Erk, Nevin
通讯作者：
Erk, Nevin