Porphyromonas spp., Fusobacterium spp., and Bacteroides spp. dominate microbiota in the course of macropod progressive periodontal disease.
Porphyromonas spp., Fusobacterium spp., and Bacteroides spp. dominate microbiota in the course of macropod progressive periodontal disease.
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DOI：
10.1038/s41598-021-97057-1
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发表时间：
2021-09-07
影响因子：
4.6
通讯作者：
Trott DJ
中科院分区：
文献类型：
--
作者：
Yip S;Dehcheshmeh MM;McLelland DJ;Boardman WSJ;Saputra S;Ebrahimie E;Weyrich LS;Bird PS;Trott DJ
大足动物进行性牙周病（MPPD）是一种坏死性、多微生物性、炎症性疾病，常见于圈养大足动物。MPPD的特征是牙龈炎与牙菌斑形成相关，其进展为牙周炎，然后发展为下颌骨或上颌骨骨髓炎。然而，这种疾病的潜在微生物原因仍然知之甚少。在这项研究中，我们收集了在南澳大利亚阿德莱德和Monarto动物园接受临床检查的22只圈养巨足科和巨足科个体的27个口腔菌斑样本和相关临床记录（健康15例，牙龈炎7例，牙周炎-骨髓炎5例）。使用Illumina Miseq对16S核糖体RNA基因的V3-V4区域进行测序，以探索MPPD与这些动物口腔细菌之间的联系。利用置换多元方差分析（Permutational Multivariate Analysis of Variance， PERMANOVA）检测牙周炎-骨髓炎病例与健康样本的微生物群组成差异（p值经Bonferroni校正< 0.01），以及牙龈炎病例与健康样本的微生物群组成差异（p值经Bonferroni校正< 0.05）。利用线性判别分析效应大小（LEfSe; p = < 0.05），还发现与健康个体相比，MPPD动物中卟啉单胞菌、梭杆菌和拟杆菌群的数量过多。MPPD样品中也检测到desulfomicroum丰度增加（LEfSe; p < 0.05），这可能反映了疾病进展的差异。这是圈养大型足类动物中MPPD的首次微生物群分析，这些结果支持MPPD的多微生物发病机制，表明支撑MPPD的微生物相互作用可能比以前文献记载的更复杂。
Macropod progressive periodontal disease (MPPD) is a necrotizing, polymicrobial, inflammatory disease commonly diagnosed in captive macropods. MPPD is characterized by gingivitis associated with dental plaque formation, which progresses to periodontitis and then to osteomyelitis of the mandible or maxilla. However, the underlying microbial causes of this disease remain poorly understood. In this study, we collected 27 oral plaque samples and associated clinical records from 22 captive Macropodidae and Potoroidae individuals that were undergoing clinical examination at Adelaide and Monarto Zoos in South Australia (15 healthy, 7 gingivitis and 5 periodontitis-osteomyelitis samples). The V3-V4 region of the 16S ribosomal RNA gene was sequenced using an Illumina Miseq to explore links between MPPD and oral bacteria in these animals. Compositional differences were detected between the microbiota of periodontitis-osteomyelitis cases compared to healthy samples (p-value with Bonferroni correction < 0.01), as well as gingivitis cases compared to healthy samples (p-value with Bonferroni correction < 0.05) using Permutational Multivariate Analysis of Variance (PERMANOVA). An overabundance of Porphyromonas, Fusobacterium, and Bacteroides taxa was also identified in animals with MPPD compared to healthy individuals using linear discriminant analysis effect size (LEfSe; p = < 0.05). An increased abundance of Desulfomicrobium also was detected in MPPD samples (LEfSe; p < 0.05), which could potentially reflect differences in disease progression. This is the first microbiota analysis of MPPD in captive macropods, and these results support a polymicrobial pathogenesis of MPPD, suggesting that the microbial interactions underpinning MPPD may be more complex than previously documented.
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影响因子：
6.4
作者：
Jorth P;Turner KH;Gumus P;Nizam N;Buduneli N;Whiteley M
通讯作者：
Whiteley M
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BEIGHTON, D;MILLER, WA
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