Location, Isolation, and Identification of Mesenchymal Stem Cells from Adult Human Sweat Glands.
Location, Isolation, and Identification of Mesenchymal Stem Cells from Adult Human Sweat Glands.
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DOI：
10.1155/2018/2090276
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发表时间：
2018
影响因子：
4.3
通讯作者：
Fu X
中科院分区：
文献类型：
--
作者：
Ma Y;Li M;Liu J;Pang C;Zhang J;Li Y;Fu X
汗腺（SG）几乎遍布人体的整个表面，对体温调节至关重要。组织特异性干细胞（tissue-specific stem cells，TSSCs）具有遗传稳定性和分化能力，是理想的SGs再生候选细胞。在此，我们尝试分离源自成人汗腺（ahSG）的TSSC。通过H&E染色、双重免疫荧光染色、透射电子显微镜（TEM）和免疫TEM定位和鉴定ahSGs。我们发现了一群具有干细胞特征（SGSC）的细胞，位于螺线管球分泌部分的基底肌上皮细胞中。SGSC表达α-平滑肌肌动蛋白（α-SMA），具有间充质干细胞（MSCs）的典型特征，CD 44、CD 73、CD 90和CD 105抗原表达阳性，具有向成骨细胞和脂肪细胞多向分化的潜能。此外，分离的α-SMA阳性细胞在第12代仍保持稳定的表型和增殖周期。这是首次报道从ahSG中成功分离MSC样细胞，其可能有助于伤口修复和SG再生。
Sweat glands (SGs) are spread over almost the entire surface of the human body and are essential for thermoregulation. Theoretically, tissue-specific stem cells (TSSCs) are excellent candidate cells for the regeneration of SGs due to their genetic stability and differentiation ability. Herein, we attempted to isolate TSSCs derived from adult human sweat glands (ahSGs). ahSGs were localized and identified by H&E staining, double immunofluorescence staining, transmission electron microscope (TEM), and immuno-TEM. We found a population of cells with stem cell characteristics (SGSCs), located in basal myoepithelial cells of the secretory portion of the solenoid bulb. The SGSCs expressed alpha-smooth muscle actin (α-SMA) and showed the typical characteristics of mesenchymal stem cells (MSCs), with a positive antigen profile for CD44, CD73, CD90, and CD105, and had the multilineage differentiation potential to osteoblasts and adipocytes. In addition, the isolated α-SMA positive cells remained stably phenotypic and proliferative cycles at passage 12. This is the first report of successful isolation of MSC-like cells from ahSGs, which may contribute to wound repair and SG regeneration.
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DOI：
10.1126/science.1242281
发表时间：
2014-06-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Blanpain C;Fuchs E
通讯作者：
Fuchs E
影响因子：
5.6
作者：
Bose A;Teh MT;Mackenzie IC;Waseem A
通讯作者：
Waseem A
影响因子：
6.1
作者：
Wang Yao;Liu Zhi-yue;Fu Xiao-bing
通讯作者：
Fu Xiao-bing
影响因子：
5.4
作者：
Gao Y;Li M;Zhang X;Bai T;Chi G;Liu JY;Li Y
通讯作者：
Li Y
影响因子：
7.7
作者：
Abdi R;Fiorina P;Adra CN;Atkinson M;Sayegh MH
通讯作者：
Sayegh MH