Mechanistic Insights Into the Cross-Feeding of Ruminococcus gnavus and Ruminococcus bromii on Host and Dietary Carbohydrates.
Mechanistic Insights Into the Cross-Feeding of Ruminococcus gnavus and Ruminococcus bromii on Host and Dietary Carbohydrates.
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DOI：
10.3389/fmicb.2018.02558
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发表时间：
2018
影响因子：
5.2
通讯作者：
Juge N
中科院分区：
文献类型：
--
作者：
Crost EH;Le Gall G;Laverde-Gomez JA;Mukhopadhya I;Flint HJ;Juge N
膳食和宿主聚糖塑造了人类肠道微生物群的组成，其中关键的碳水化合物降解物种在维持肠道微生物群落的结构和功能方面发挥着关键作用。在这里，我们集中在两个主要的人类肠道共生菌，粘蛋白降解菌Ruminococcus gavus ATCC 29149和R。bromii L2-63是在人结肠中降解抗性淀粉（RS）的关键种。利用厌氧单菌和共培养的R. bromii和R. gnavus生长在粘蛋白或淀粉为唯一碳源，我们发现，淀粉降解R。bromii支持R. gnavus，而R. bromii没有从R. gnavus。此外，我们分析了生长（定量PCR），代谢产物的生产（1H NMR分析），和细菌转录反应（RNA-Seq）的R。在存在或不存在R. gnavus。在淀粉共培养发酵中，1H NMR分析表明，R. gnavus从R.溴在淀粉降解后产生乙酸盐、甲酸盐和乳酸盐作为主要发酵终产物。对淀粉（SS和RS）的差异表达分析（DESeq 2）表明，R.溴素诱导的R. gnavus的麦芽糖转运蛋白和麦芽糖磷酸化酶等代谢相关酶基因的转录反应，与R. gnavus利用R.溴化淀粉降解产物。在RS共培养中，R.与单一培养物相比，Bromii表现出色氨酸（Trp）生物合成基因的诱导显著增加和维生素B12（VitB 12）依赖的甲硫氨酸生物合成的减少，表明Trp和VitB 12的可用性在R. gnavus。总之，这项研究表明，R。bromii和R. gnavus对RS的作用，表明在体内，R.栖息在粘液生态位的gavus种群可以通过供应到达结肠的不可消化的碳水化合物如RS来调节。
Dietary and host glycans shape the composition of the human gut microbiota with keystone carbohydrate-degrading species playing a critical role in maintaining the structure and function of gut microbial communities. Here, we focused on two major human gut symbionts, the mucin-degrader Ruminococcus gnavus ATCC 29149, and R. bromii L2-63, a keystone species for the degradation of resistant starch (RS) in human colon. Using anaerobic individual and co-cultures of R. bromii and R. gnavus grown on mucin or starch as sole carbon source, we showed that starch degradation by R. bromii supported the growth of R. gnavus whereas R. bromii did not benefit from mucin degradation by R. gnavus. Further we analyzed the growth (quantitative PCR), metabolite production (1H NMR analysis), and bacterial transcriptional response (RNA-Seq) of R. bromii cultured with RS or soluble starch (SS) in the presence or absence of R. gnavus. In co-culture fermentations on starch, 1H NMR analysis showed that R. gnavus benefits from transient glucose and malto-oligosaccharides released by R. bromii upon starch degradation, producing acetate, formate, and lactate as main fermentation end-products. Differential expression analysis (DESeq 2) on starch (SS and RS) showed that the presence of R. bromii induced changes in R. gnavus transcriptional response of genes encoding several maltose transporters and enzymes involved in its metabolism such as maltose phosphorylase, in line with the ability of R. gnavus to utilize R. bromii starch degradation products. In the RS co-culture, R. bromii showed a significant increase in the induction of tryptophan (Trp) biosynthesis genes and a decrease of vitamin B12 (VitB12)-dependent methionine biosynthesis as compared to the mono-culture, suggesting that Trp and VitB12 availability become limited in the presence of R. gnavus. Together this study showed a direct competition between R. bromii and R. gnavus on RS, suggesting that in vivo, the R. gnavus population inhabiting the mucus niche may be modulated by the supply of non-digestible carbohydrates reaching the colon such as RS.
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DOI：
10.1038/nrmicro3552
发表时间：
2016-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Donaldson GP;Lee SM;Mazmanian SK
通讯作者：
Mazmanian SK
影响因子：
64.8
作者：
通讯作者：
--
聚糖代谢如何塑造人类肠道微生物群。
DOI：
10.1038/nrmicro2746
发表时间：
2012-04-11
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1096/fj.12-221416
发表时间：
2013-06
期刊：
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
影响因子：
--
作者：
Gunning AP;Kirby AR;Fuell C;Pin C;Tailford LE;Juge N
通讯作者：
Juge N
DOI：
10.1007/s10482-018-1040-x
发表时间：
2018-06
期刊：
Antonie van Leeuwenhoek
影响因子：
--
作者：
Chia LW;Hornung BVH;Aalvink S;Schaap PJ;de Vos WM;Knol J;Belzer C
通讯作者：
Belzer C