MERS-CoV infection in humans is associated with a pro-inflammatory Th1 and Th17 cytokine profile.
MERS-CoV infection in humans is associated with a pro-inflammatory Th1 and Th17 cytokine profile.
复制标题
DOI：
10.1016/j.cyto.2018.01.025
复制
发表时间：
2018-04
期刊：
影响因子：
3.8
通讯作者：
Suliman BA
中科院分区：
文献类型：
--
作者：
Mahallawi WH;Khabour OF;Zhang Q;Makhdoum HM;Suliman BA
MERS由一种名为MERS冠状病毒的单链RNA病毒引起。MERS-CoV感染引起I型和II型干扰素应答。MERS-CoV感染患者显示出突出的Th1和Th17细胞因子谱。MERS-CoV感染诱导IL-10的表达。中东呼吸综合征冠状病毒（MERS-CoV）已被认为是对人类的高致病性病毒，其感染呼吸道并与高发病率和死亡率相关。动物模型的研究表明，MERS-CoV感染诱导强烈的炎症反应，这可能与疾病的严重程度有关。显示MERS-CoV感染急性期人体细胞因子谱的数据有限。在这项研究中，我们分析了与健康对照组（n = 13）相比，确认MERS-CoV感染患者（n = 7）血浆样本中细胞因子反应的概况。细胞因子谱，包括辅助性T细胞（Th）1，Th2和Th17反应，使用流式细胞仪珠阵列（CBA）进行分析。在MERS-CoV感染患者中清楚地观察到显著的促炎性Th1和Th17应答，与对照组相比，IFN-γ、TNF-α、IL-15和IL-17的浓度显著增加。MERS-CoV感染患者IL-12表达水平与健康对照组无差异，尽管IFN-α 2和IFN-γ水平显著升高（P <0.01）。IL-2、IL-4、IL-5、IL-13和TGF-α水平无变化（P> 0.05）。我们的研究结果表明，在人类MERS-CoV感染的急性期有显著的促炎细胞因子反应。
MERS is caused by a single-stranded RNA virus named MERS-corona virus. Infection with MERS-CoV elicits a type-I and type-II interferon response. MERS-CoV infected patients show a prominent Th1 and Th17 cytokine profile. MERS-CoV infection induces the expression of IL-10. The Middle East respiratory syndrome coronavirus (MERS-CoV) has been recognized as a highly pathogenic virus to humans that infects the respiratory tract and is associated with high morbidity and mortality. Studies in animal models suggest that MERS-CoV infection induces a strong inflammatory response, which may be related to the severity of disease. Data showing the cytokine profiles in humans during the acute phase of MERS-CoV infection are limited. In this study, we have analyzed the profile of cytokine responses in plasma samples from patients with confirmed MERS-CoV infections (n = 7) compared to healthy controls (n = 13). The cytokine profiles, including T helper (Th) 1, Th2 and Th17 responses, were analyzed using cytometric bead array (CBA). A prominent pro-inflammatory Th1 and Th17 response was clearly seen in patients with MERS-CoV infection, with markedly increased concentrations of IFN-γ, TNF-α, IL-15 and IL-17 compared to controls. IL-12 expression levels showed no difference between patients with MERS-CoV infection and the healthy controls despite the significantly increased levels of IFN-α2 and IFN-γ (P < .01). No changes were observed in the levels of IL-2, IL-4, IL-5, IL-13, and TGF-α (P > .05). Our results demonstrate a marked pro-inflammatory cytokine response during the acute phase of MERS-CoV infection in humans.
登录
查看更多内容
影响因子：
13.2
作者：
Jin, Wei;Dong, Chen
通讯作者：
Dong, Chen
影响因子：
7.3
作者：
Ali AK;Nandagopal N;Lee SH
通讯作者：
Lee SH
DOI：
10.1073/pnas.1519235113
发表时间：
2016-08-09
影响因子：
11.1
作者：
Cauchemez, Simon;Nouvellet, Pierre;Ferguson, Neil M.
通讯作者：
Ferguson, Neil M.
影响因子：
15.9
作者：
Blackburn, Shawn D.;Wherry, E. John
通讯作者：
Wherry, E. John
DOI：
10.1056/nejmoa1306742
发表时间：
2013-08-01
期刊：
The New England journal of medicine
影响因子：
--
作者：
Assiri A;McGeer A;Perl TM;Price CS;Al Rabeeah AA;Cummings DA;Alabdullatif ZN;Assad M;Almulhim A;Makhdoom H;Madani H;Alhakeem R;Al-Tawfiq JA;Cotten M;Watson SJ;Kellam P;Zumla AI;Memish ZA;KSA MERS-CoV Investigation Team
通讯作者：
KSA MERS-CoV Investigation Team