Neurogenesis of retinal ganglion cells is not essential to visual functional recovery after optic nerve injury in adult zebrafish.
Neurogenesis of retinal ganglion cells is not essential to visual functional recovery after optic nerve injury in adult zebrafish.
复制标题
视网膜神经节细胞的神经发生对于成年斑马鱼视神经损伤后的视觉功能恢复并不重要
DOI：
10.1371/journal.pone.0057280
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Hu B
中科院分区：
文献类型：
--
作者：
Zou S;Tian C;Ge S;Hu B
斑马鱼中枢神经系统(CNS)具有很强的神经再生能力，能够在视神经损伤(ONI)后完全恢复视觉功能。然而，视网膜神经节细胞(RGC)的神经发生是否有助于功能恢复仍存在争议。我们对不同ONI模型中的RGCs进行了定量分析，结果表明，在视神经挤压(ONC)模型中，几乎所有的RGCs存活，而在视神经切断(ONT)模型中，90%以上的RGCs在前2周存活，7周后仍有75%的RGCs存活。顶盖逆行标记显示了惊人的再生速度，在ONC和ONT模型的第一周，分别有90%和50%以上的RGC再生到顶盖。4周后再生的RGCs数与对照组无明显差异。至于神经发生，无论是双标记法还是BrdU标记法都很少检测到新生RGCs。由于视网膜节细胞很少死亡，小胶质细胞数量在伤后3天出现暂时性增加，在伤后14天减少。伤后5周，视网膜髓鞘结构完整，视运动反应(OMR)显示视功能恢复。综上所述，我们的结果直接表明RGC存活和轴突再生与ONI后成年斑马鱼的功能恢复有关。
Zebrafish central nervous system (CNS) possesses a strong neural regeneration ability to restore visual function completely after optic nerve injury (ONI). However, whether neurogenesis of retinal ganglion cell (RGC) contributes to functional recovery remains controversial. Our quantitative analysis of RGCs in different ONI models showed that almost all RGCs survived in optic nerve crush (ONC) model; while over 90% of RGCs survived in the first 2 weeks with 75% remaining after 7 weeks in optic nerve transection (ONT) model. Retrograde labeling from tectum revealed a surprising regeneration rate, with over 90% and over 50% of RGCs regrowing axons to tectum at the first week in ONC and ONT model respectively. In the latter one, the number of regenerative RGCs after 4 weeks had no significant difference from the control group. As for neurogenesis, newborn RGCs were rarely detected either by double retrograde labeling or BrdU marker. Since few RGCs died, microglia number showed a temporary increase at 3 days post injury (dpi) and a decrease at 14 dpi. Finally, myelin structure within retina kept integrity and optomotor response (OMR) test demonstrated visual functional restoration at 5 weeks post injury (wpi). In conclusion, our results have directly shown that RGC survival and axon regrowth are responsible for functional recovery after ONI in adult zebrafish.
登录
查看更多内容
影响因子：
3.4
作者：
Germain, F;Calvo, M;de la Villa, P
通讯作者：
de la Villa, P
影响因子：
14.8
作者：
Iovine, M. Katryn
通讯作者：
Iovine, M. Katryn
DOI：
10.1023/b:mics.0000006854.18378.fc
发表时间：
2003-01-01
期刊：
Methods in Cell Science
影响因子：
--
作者：
Liu, Q.;Londraville, R. L.
通讯作者：
Londraville, R. L.
影响因子：
2.9
作者：
Kaneda, Manabu;Nagashima, Mikiko;Kato, Satoru
通讯作者：
Kato, Satoru
影响因子：
6.2
作者：
Brösamle, C;Halpern, ME
通讯作者：
Halpern, ME