Adherence to streptococci facilitates Fusobacterium nucleatum integration into an oral microbial community.
Adherence to streptococci facilitates Fusobacterium nucleatum integration into an oral microbial community.
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DOI：
10.1007/s00248-011-9989-2
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发表时间：
2012-04
影响因子：
3.6
通讯作者：
Lux, Renate
中科院分区：
文献类型：
--
作者：
He, Xuesong;Hu, Wei;Kaplan, Christopher W.;Guo, Lihong;Shi, Wenyuan;Lux, Renate
多物种口腔微生物群落的发展涉及复杂的物种内和物种间的各种水平的相互作用。粘附于常驻细菌或生物膜基质并克服群落抗性的能力是决定细菌是否可以整合到群落中的关键因素之一。在这项研究中，我们专注于社区整合的具核梭杆菌，一种流行的革兰氏阴性口腔细菌物种，被认为是口腔社区的重要成员，由于其能够坚持革兰氏阳性以及革兰氏阴性菌。这种与各种不同物种的相互作用被认为有助于建立多物种口腔微生物群落。然而，迄今为止，大多数实验都集中在两个物种之间的物理粘附通过体外共聚集测定，而基于社区的F。核质进入多物种微生物群落仍有待研究。在这项研究中，我们证明了使用一个建立在体外小鼠口腔微生物群（O-混合物），F。在加入O-混合物后，由于口腔群落对过氧化氢（H2 O2）产生的细胞接触依赖性诱导，核质的浓度显著降低。有趣的是，当F.在添加前，允许具核质粘附到其已知的相互作用伴侣物种（例如血链球菌）上。此外，这种聚集体形成依赖性保护在F。核质突变株Δ Fn 1526，其不能结合许多革兰氏阳性物种。更重要的是，这种保护作用在F.有核质进入人类唾液微生物群落（S-mix）。这些结果支持了F.具核质能够掩盖被产生H2 O2的口腔群落成员识别的表面组分。这种逃避策略防止了拮抗性口腔细菌的检测，并允许整合到发育中的口腔微生物群落中。
The development of multispecies oral microbial communities involves complex intra- and interspecies interactions at various levels. The ability to adhere to the resident bacteria or the biofilm matrix and overcome community resistance are among the key factors that determine whether a bacterium can integrate into a community. In this study, we focus on community integration of Fusobacterium nucleatum, a prevalent Gram-negative oral bacterial species that is considered an important member of the oral community due to its ability to adhere to Gram-positive as well as Gram-negative species. This interaction with a variety of different species is thought to facilitate the establishment of multispecies oral microbial community. However, the majority of experiments thus far has focused on the physical adherence between two species as measured by in vitro co-aggregation assays, while the community-based effects on the integration of F. nucleatum into multispecies microbial community remains to be investigated. In this study, we demonstrated using an established in vitro mice oral microbiota (O-mix) that the viability of F. nucleatum was significantly reduced upon addition to the O-mix due to cell contact-dependent induction of hydrogen peroxide (H2O2) production by oral community. Interestingly, this inhibitory effect was significantly alleviated when F. nucleatum was allowed to adhere to its known interacting partner species (such as Streptococcus sanguinis) prior to addition. Furthermore, this aggregate formation-dependent protection was absent in the F. nucleatum mutant strain ΔFn1526 that is unable to bind to a number of Gram-positive species. More importantly, this protective effect was also observed during integration of F. nucleatum into a human salivary microbial community (S-mix). These results support the idea that by adhering to other oral microbes, such as streptococci, F. nucleatum is able to mask the surface components that are recognized by H2O2 producing oral community members. This evasion strategy prevents detection by antagonistic oral bacteria and allows integration into the developing oral microbial community.
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