A new variant of the capsule 3 cluster occurs in Streptococcus pneumoniae from deceased wild chimpanzees.
A new variant of the capsule 3 cluster occurs in Streptococcus pneumoniae from deceased wild chimpanzees.
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DOI：
10.1371/journal.pone.0025119
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Hakenbeck R
中科院分区：
文献类型：
--
作者：
Denapaite D;Hakenbeck R
最近，通过对从科特迪瓦塔霍河国家公园获得的死亡类人猿样本进行DNA序列分析，证实了在先前患有呼吸系统疾病的死亡野生黑猩猩中存在新的肺炎链球菌克隆。为了拓宽我们对这些肺炎球菌克隆与人类克隆的相关性的理解，现在已经表征了负责荚膜多糖（CPS）生物合成的基因位点。PCR片段的DNA序列分析鉴定了一个名为cps 3 Tax 2的簇，该簇包含4个血清型3 CPS的典型基因，但缺少一个≥ 2kb的5′-区域，该区域在其他cps 3基因座中退化，并且不是3型CPS生物合成所必需的。CPS 3由包含葡萄糖和葡萄糖醛酸（GlcUA）的简单二糖重复单元组成。负责GlcUA合成的两个基因ugd和编码3型合酶的wchE是CPS 3生物合成所必需的，而galU和3′-截短的基因pgm由于基因组中其他地方存在同源物而不是必需的。cps 3基因座的DNA序列与其它cps 3基因座的DNA序列差异较大。此外，基因pgm Taxis代表在密码子179处具有无义突变的全长形式。将包括启动子区的两个基因ugd TaxB和wchE TaxB转化到未包囊的实验室菌株S中。pneumoniae R6.表达3型荚膜多糖的转化体很容易获得，证明基因产物是有功能的。总之，数据表明，cps 3 Tac独立于其他cps 3基因座进化，表明存在特异性血清型3S。肺炎克隆的地方性的塔霍河国家公园地区。
The presence of new Streptococcus pneumoniae clones in dead wild chimpanzees from the Taï National Park, Côte d'Ivoire, with previous respiratory problems has been demonstrated recently by DNA sequence analysis from samples obtained from the deceased apes. In order to broadenour understanding on the relatedness of these pneumococcal clones to those from humans, the gene locus responsible for biosynthesis of the capsule polysaccharide (CPS) has now been characterized. DNA sequence analysis of PCR fragments identified a cluster named cps3Taï containing the four genes typical for serotype 3 CPS, but lacking a 5′-region of ≥2 kb which is degenerated in other cps3 loci and not required for type 3 biosynthesis. CPS3 is composed of a simple disaccharide repeat unit comprising glucose and glucuronic acid (GlcUA). The two genes ugd responsible for GlcUA synthesis and wchE encoding the type 3 synthase are essential for CPS3 biosynthesis, whereas both, galU and the 3′-truncated gene pgm are not required due to the presence of homologues elsewhere in the genome. The DNA sequence of cps3Taï diverged considerably from those of other cps3 loci. Also, the gene pgm Taï represents a full length version with a nonsense mutation at codon 179. The two genes ugd Taï and wchE Taï including the promoter region were transformed into a nonencapsulated laboratory strain S. pneumoniae R6. Transformants which expressed type 3 capsule polysaccharide were readily obtained, documenting that the gene products are functional. In summary, the data indicate that cps3Taï evolved independent from other cps3 loci, suggesting the presence of specialized serotype 3 S. pneumoniae clones endemic to the Taï National Park area.
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DOI：
10.1084/jem.181.3.973
发表时间：
1995-03-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Dillard JP;Vandersea MW;Yother J
通讯作者：
Yother J
影响因子：
9.4
作者：
BENSON, CE;SWEENEY, CR
通讯作者：
SWEENEY, CR
影响因子：
5.1
作者：
Domenech, Mirian;Garcia, Ernesto;Moscoso, Miriam
通讯作者：
Moscoso, Miriam
研究肺炎球菌类型的诱导物质转化的化学性质：通过从III型肺炎球菌分离出的脱氧核糖核酸分数诱导转化。
DOI：
10.1084/jem.79.2.137
发表时间：
1944-02-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Avery OT;Macleod CM;McCarty M
通讯作者：
McCarty M
DOI：
10.1084/jem.54.1.73
发表时间：
1931-06-30
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Avery OT;Dubos R
通讯作者：
Dubos R