Evaluation of Urinary Big Endothelin-1 in Feline Spontaneous CKD.
Evaluation of Urinary Big Endothelin-1 in Feline Spontaneous CKD.
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DOI：
10.3390/ani10112144
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发表时间：
2020-11-18
期刊：
影响因子：
--
通讯作者：
Scarpa P
中科院分区：
文献类型：
--
作者：
Giraldi M;Paltrinieri S;Piazza C;Scarpa P
慢性肾脏病是老年猫的常见和进行性疾病。这是宠物痛苦和主人费用的原因。早期诊断和非侵入性评估肾损伤的生物标志物在研究和临床实践中肯定是有用的。在这项研究中，我们评估了受慢性肾脏疾病影响的猫尿液中的生物标志物大内皮素-1。大内皮素-1是与炎症和压力调节相关的分子，以前没有在肾病猫中进行过评估。我们发现，在疾病晚期和蛋白尿（肾损害的标志物）患者中，尿大内皮素增加。尽管如此，大内皮素1似乎不是疾病进展的有用生物标志物。根据这项初步研究的结果，我们建议这种生物标志物为未来的研究猫肾脏疾病。内皮素-1（ET-1）系统与慢性肾脏病（CKD）的发生和进展有关。没有关于猫尿液中大ET-1的信息。本研究的目的是评估尿大内皮素-1（bigET-1）是否与猫CKD相关。从13只有患CKD风险的健康猫和22只患有不同国际肾脏利益协会（IRIS）分期（1-4）的CKD猫中前瞻性收集了60份尿样。使用市售ELISA测量尿bigET-1。根据IRIS的建议，将尿肌酐标准化的BigET-1（bigET-1：UC）在分期和亚分期之间进行比较，并与血清肌酐浓度、蛋白尿和血压相关。比较了保持稳定的猫和12个月后进展的猫在入选时的BigET-1：UC。与所有其他阶段相比，IRIS 3-4期猫的BigET-1：UC显著更高（p = 0.002）（中位数：21.9;范围：1.88-55.6），蛋白尿猫（n = 8，中位数：11.0;范围：0.00-46.4）显著高于非蛋白尿猫（n = 38，中位数：0.33;范围：0.00-55.6）（p = 0.029）。BigET-1：UC与CKD进展无关。尿bigET-1在CKD晚期和蛋白尿患者中增加，表明ET-1可能指示猫CKD的严重程度。
Chronic kidney disease is a common and progressive disease of elderly cats. It is a cause of pet suffering and owner expense. Biologic biomarkers for early diagnosis and for noninvasive evaluation of kidney damage are certainly useful in both research and clinical practice. In this study, we evaluated the biomarker big endotelin-1 in the urine of cats affected with chronic kidney disease. Big endothelin-1 is molecule linked to inflammation and pressure regulation, and it was not previously evaluated in nephropathic cats. We found that urinary big endothelin was increased in patients at late stage of the disease and in patients with proteinuria (a marker of kidney damage). Despite that, big endothelin 1 seemed not to be a useful biomarker for disease progression. According to results of this preliminary study, we suggest this biomarker for future research on feline kidney disease. The endothelin-1 (ET-1) system has been implicated in the development and progression of chronic kidney disease (CKD). No information on big ET-1 in feline urine is available. The purpose of this study was to evaluate if urinary big endothelin-1 (bigET-1) is associated with feline CKD. Sixty urine samples were prospectively collected from 13 healthy cats at risk of developing CKD and 22 cats with CKD of different International Renal Interest Society (IRIS) stages (1–4). Urinary bigET-1 was measured using a commercially available ELISA. BigET-1 normalized to urine creatinine (bigET-1:UC) was compared amongst stages and substages, as proposed by IRIS, and correlated with serum creatinine concentration, proteinuria and blood pressure. BigET-1:UC at the time of inclusion was compared between cats that remained stable and cats that progressed after 12 months. BigET-1:UC was significantly higher (p = 0.002) in cats at IRIS stages 3–4 (median: 21.9; range: 1.88–55.6), compared to all other stages, and in proteinuric (n = 8, median: 11.0; range: 0.00–46.4) compared with nonproteinuric cats (n = 38 median: 0.33; range: 0.00–55.6) (p = 0.029). BigET-1:UC was not associated with CKD progression. Urinary bigET-1 increased in advanced stages of CKD and in proteinuric patients, suggesting that ET-1 may be indicative of the severity of feline CKD.
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