Molecular characterization of Staphylococcus aureus from outpatients in the Caribbean reveals the presence of pandemic clones.
Molecular characterization of Staphylococcus aureus from outpatients in the Caribbean reveals the presence of pandemic clones.
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来自加勒比海门诊病人的金黄色葡萄球菌的分子表征揭示了大流行克隆的存在。
DOI：
10.1007/s10096-011-1339-2
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发表时间：
2012-04
影响因子：
4.5
通讯作者：
Lowy, F. D.
中科院分区：
文献类型：
--
作者：
Uhlemann, A-C;Dumortier, C.;Hafer, C.;Taylor, B. S.;Sanchez E, J.;Rodriguez-Taveras, C.;Leon, P.;Rojas, R.;Olive, C.;Lowy, F. D.
金黄色葡萄球菌感染继续构成全球公共卫生问题。通常情况下，这种流行是由金黄色葡萄球菌的单个克隆在一个地理区域内的成功传播所推动的，但国际旅行已被认为是金黄色葡萄球菌感染的一个潜在风险因素。为了研究加勒比地区金黄色葡萄球菌感染的分子流行病学，我们收集了来自多米尼加共和国(n=112)和马提尼克岛(n=143)社区感染的甲氧西林敏感金黄色葡萄球菌(MSSA)和耐甲氧西林金黄色葡萄球菌(MRSA)分离株。用脉冲场凝胶电泳法(PFGE)、SpA分型和多位点序列分型(MLST)相结合的方法对分离株进行鉴定。在马提尼克岛，MRSA感染(n=56)主要由t304-st8菌株(n=44)引起，而MSSA分离株来自不同的遗传背景。在来自多米尼加共和国的22株MRSA中，ST5、ST30和ST72占主导地位，而ST30 T665-PVL+(30/90)占相当数量的MSSA感染。尽管这两个国家的样本收集存在流行病学差异，但相当数量的MSSA感染(~10%)是由每个地点的ST5和ST398分离株引起的。进一步的系统发育分析表明，这两个国家存在共同的血统，随后每个地点最近出现了独特的基因多样性。我们的发现还表明，加勒比地区的国际金黄色葡萄球菌菌株经常进口和交换。
Staphylococcus aureus infections continue to pose a global public health problem. Frequently, this epidemic is driven by the successful spread of single S. aureus clones within a geographic region, but international travel has been recognized as a potential risk factor for S. aureus infections. To study the molecular epidemiology of S. aureus infections in the Caribbean, a major international tourist destination, we collected methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) isolates from community-onset infections in the Dominican Republic (n=112) and Martinique (n=143). Isolates were characterized by a combination of pulsed-field gel electrophoresis (PFGE), spa typing, and multilocus sequence typing (MLST) typing. In Martinique, MRSA infections (n=56) were mainly caused by t304-ST8 strains (n=44), whereas MSSA isolates were derived from genetically diverse backgrounds. Among MRSA strains (n=22) from the Dominican Republic, ST5, ST30, and ST72 predominated, while ST30 t665-PVL+ (30/90) accounted for a substantial number of MSSA infections. Despite epidemiological differences in sample collections from both countries, a considerable number of MSSA infections (~10%) were caused by ST5 and ST398 isolates at each site. Further phylogenetic analysis suggests the presence of lineages shared by the two countries, followed by recent genetic diversification unique to each site. Our findings also imply the frequent import and exchange of international S. aureus strains in the Caribbean.
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影响因子：
1.6
作者：
Kaneko, J;Muramoto, K;Kamio, Y
通讯作者：
Kamio, Y
影响因子：
14.2
作者：
Baranovich, T.;Zaraket, H.;Suzuki, H.
通讯作者：
Suzuki, H.
影响因子：
11.8
作者：
Bhat M;Dumortier C;Taylor BS;Miller M;Vasquez G;Yunen J;Brudney K;Sánchez-E J;Rodriguez-Taveras C;Rojas R;Leon P;Lowy FD
通讯作者：
Lowy FD
影响因子：
3.7
作者：
Cuny C;Nathaus R;Layer F;Strommenger B;Altmann D;Witte W
通讯作者：
Witte W
影响因子：
6.4
作者：
Rasigade, Jean-Philippe;Laurent, Frederic;Tristan, Anne
通讯作者：
Tristan, Anne