Static and Dynamic Measurement of Dopamine Adsorption in Carbon Fiber Microelectrodes Using Electrochemical Impedance Spectroscopy.
Static and Dynamic Measurement of Dopamine Adsorption in Carbon Fiber Microelectrodes Using Electrochemical Impedance Spectroscopy.
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DOI：
10.1021/acs.analchem.7b04692
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发表时间：
2018-02-06
影响因子：
7.4
通讯作者：
Cunci L
中科院分区：
文献类型：
--
作者：
Rivera-Serrano N;Pagan M;Colón-Rodríguez J;Fuster C;Vélez R;Almodovar-Faria J;Jiménez-Rivera C;Cunci L
本研究首次将电化学阻抗谱用于多巴胺在碳纤维微电极上的吸附研究。为了展示概念验证，在相对于Ag的-0.4至0.8 V的电位范围内进行静态和动态测量|AgCl来证明这种技术的多功能性，以研究多巴胺，而不需要其氧化。我们使用电化学阻抗谱和单频电化学阻抗来测量低至1 nM的不同浓度的多巴胺。此外，微电极表面的电容被发现由于多巴胺的吸附，这是依赖于它的浓度降低。研究了溶解氧和表面电化学氧化对多巴胺检测的影响。制备了未氧化和氧化碳纤维微电极，并通过光学显微镜、扫描电子显微镜、循环伏安法和电化学阻抗谱对其进行了表征。为了更好地进行测量，获得了电极的最佳工作参数，如频率和电压。在不同浓度、电压和频率下测定了多巴胺的电化学阻抗。最后，使用流动池和单频阻抗进行动态实验，以研究多巴胺的连续和实时测量。
In this study, electrochemical impedance spectroscopy was used for the first time to study the adsorption of dopamine in carbon fiber microelectrodes. In order to show a proof-of-concept, static and dynamic measurements were taken at potentials ranging from −0.4 to 0.8 V vs Ag|AgCl to demonstrate the versatility of this technique to study dopamine without the need of its oxidation. We used electrochemical impedance spectroscopy and single frequency electrochemical impedance to measure different concentrations of dopamine as low as 1 nM. Moreover, the capacitance of the microelectrodes surface was found to decrease due to dopamine adsorption, which is dependent of its concentration. The effect of dissolved oxygen and electrochemical oxidation of the surface in the detection of dopamine was also studied. Non-oxidized and oxidized carbon fiber microelectrodes were prepared and characterized by optical microscopy, scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. Optimum working parameters of the electrodes, such as frequency and voltage, were obtained for better measurement. Electrochemical impedance of dopamine was determined at different concentration, voltages, and frequencies. Finally, dynamic experiments were conducted using a flow cell and single frequency impedance in order to study continuous and real-time measurements of dopamine.
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影响因子：
10.6
作者：
Park, Jinwoo;Wheeler, Robert A.;Fontillas, Khristy;Keithley, Richard B.;Carelli, Regina M.;Wightman, R. Mark
通讯作者：
Wightman, R. Mark
影响因子：
4.2
作者：
Heien, MLAV;Phillips, PEM;Wightman, RM
通讯作者：
Wightman, RM
影响因子：
6.6
作者：
Wang, Ruimin;Di, Jing;Ma, Zhanfang
通讯作者：
Ma, Zhanfang
影响因子：
7.4
作者：
Bath, BD;Michael, DJ;Wightman, RM
通讯作者：
Wightman, RM
影响因子：
8.9
作者：
Yang C;Trikantzopoulos E;Nguyen MD;Jacobs CB;Wang Y;Mahjouri-Samani M;Ivanov IN;Venton BJ
通讯作者：
Venton BJ