Effects of Bacillus subtilis KN-42 on Growth Performance, Diarrhea and Faecal Bacterial Flora of Weaned Piglets.
Effects of Bacillus subtilis KN-42 on Growth Performance, Diarrhea and Faecal Bacterial Flora of Weaned Piglets.
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枯草芽孢杆菌KN-42对断奶仔猪生长性能、腹泻及粪便菌群的影响
DOI：
10.5713/ajas.2013.13737
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发表时间：
2014-08
期刊：
影响因子：
--
通讯作者：
Liang Y
中科院分区：
文献类型：
--
作者：
Hu Y;Dun Y;Li S;Zhao S;Peng N;Liang Y
本研究重点关注不同剂量的枯草芽孢杆菌KN-42对断奶仔猪生长性能、腹泻发生率、粪便细菌菌群以及粪便中乳酸杆菌和大肠杆菌相对数量的影响，以确定该菌株是否可以作为候选抗菌生长促进剂。将360头26±2日龄断奶仔猪（初始体重7.14±0.63 kg）随机分配至5个处理组（每个处理4栏，每栏18头猪）进行为期28天的试验。日粮处理为不添加任何抗菌药物的基础日粮（阴性对照；NC）、基础日粮添加120 mg/kg饲料的硫酸新霉素（阳性对照；PC）和基础日粮添加2×109（L）、4×109（M）和20×109（H）CFU/kg饲料的枯草芽孢杆菌KN-42。整个期间，PC、M和H组仔猪的平均日增重和饲料效率均高于NC组（p<0.05），所有益生菌和抗生素组的腹泻指数均低于NC组（p<0.05）。实验第 28 天，使用基于 16S rDNA 基因的方法分析粪便细菌菌群。变性梯度凝胶电泳分析结果表明，日粮中添加枯草芽孢杆菌KN-42改变了细菌群落，M组细菌多样性和条带数高于其他四组。实时聚合酶链反应分析显示，PC组和H组的乳酸菌相对数量高于NC组（p<0.05），并且日粮中添加枯草芽孢杆菌KN-42也降低了大肠杆菌的相对数量（p<0.05）。这些结果表明，日粮中添加枯草芽孢杆菌 KN-42 可以改善仔猪的生长性能和胃肠道健康。
This research focused on the effects of different doses of Bacillus subtilis KN-42 on the growth performance, diarrhea incidence, faecal bacterial flora, and the relative number of Lactobacillus and Escherichia coli in faeces of weaned piglets to determine whether the strain can serve as a candidate antimicrobial growth promoter. A total of 360 piglets (initial body weight 7.14±0.63 kg) weaned at 26±2 days of age were randomly allotted to 5 treatment groups (4 pens per treatment with 18 pigs per pen) for a 28-day trial. Dietary treatments were basal diet without any antimicrobial (negative control; NC), basal diet supplemented with 120 mg/kg feed of neomycin sulfate (positive control; PC) and basal diet supplemented with 2×109 (L), 4×109 (M) and 20×109 (H) CFU/kg feed of B. subtilis KN-42. During the overall period, average daily gain and feed efficiency of piglets were higher in groups PC, M, and H than those in group NC (p<0.05), and all probiotics and antibiotics groups had a lower diarrhea index than group NC (p<0.05). The 16S rDNA gene-based methods were used to analyze faecal bacterial flora on day 28 of experiment. The result of denaturing gradient gel electrophoresis analysis showed that supplementation of B. subtilis KN-42 to the diet changed the bacterial communities, with a higher bacterial diversity and band number in group M than in the other four groups. Real-time polymerase chain reaction analysis showed that the relative number of Lactobacillus were higher in groups PC and H than in group NC (p<0.05), and the supplemented B. subtilis KN-42 to the diet also reduced the relative number of E. coli (p<0.05). These results suggest that dietary addition of B. subtilis KN-42 can improve the growth performance and gastrointestinal health of piglets.
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影响因子：
2
作者：
Petersson, Agnes;Domig, Konrad J.;Kneifel, Wolfgang
通讯作者：
Kneifel, Wolfgang
DOI：
10.5713/ajas.2012.12110
发表时间：
2012-09
期刊：
Asian-Australasian journal of animal sciences
影响因子：
--
作者：
Aliakbarpour HR;Chamani M;Rahimi G;Sadeghi AA;Qujeq D
通讯作者：
Qujeq D
影响因子：
4.4
作者：
Lee, K. W.;Lee, S. H.;Siragusa, G. R.
通讯作者：
Siragusa, G. R.
对大量人类肠道微生物群中抗生素抗性基因进行宏基因组分析
影响因子：
16.6
作者：
Hu, Yongfei;Yang, Xi;Zhu, Baoli
通讯作者：
Zhu, Baoli
影响因子：
3.1
作者：
Jones, Sara E.;Knight, Katherine L.
通讯作者：
Knight, Katherine L.