Early differential gene expression in haemocytes from resistant and susceptible Biomphalaria glabrata strains in response to Schistosoma mansoni.
Early differential gene expression in haemocytes from resistant and susceptible Biomphalaria glabrata strains in response to Schistosoma mansoni.
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对曼森血吸虫的抗性和易感生物胶质菌株的脱瘤细胞的早期差异基因表达。
DOI：
10.1371/journal.pone.0051102
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Jones CS
中科院分区：
文献类型：
--
作者：
Lockyer AE;Emery AM;Kane RA;Walker AJ;Mayer CD;Mitta G;Coustau C;Adema CM;Hanelt B;Rollinson D;Noble LR;Jones CS
宿主裸螺（Biomphalaria glabrata）感染曼氏血吸虫（Schistosoma mansoni）的结果由它们之间发生的初始分子相互作用决定。血吸虫逃避蜗牛免疫识别以确保生存的机制尚不完全清楚，但一种可能性是血吸虫操纵了蜗牛的内部防御系统，使寄生虫得以建立感染。本研究通过使用新型5K cDNA芯片，同时比较暴露于寄生虫和对照组血吸虫抗性和敏感性菌株的血细胞中基因表达，在暴露于mansoni miracidia 2小时后，在转录组水平上对裸露白螺旋体血吸虫抗性和敏感性的性质提供了新的见解。基因表达差异，包括免疫/应激反应基因、信号转导基因和基质/粘附基因在两种蜗牛品系之间的表达差异，基因功能的不对称分布测试也发现了抗性蜗牛中免疫相关基因的表达，但在易感蜗牛中没有。基因集富集分析显示，参与线粒体电子传递、泛素生物合成和电子载体活性的基因在抗性钉螺中持续上调，而在易感钉螺中持续下调。这支持了一种假设，即抗血吸虫的蜗牛能够识别血吸虫并产生适当的防御反应，而在血吸虫易感的蜗牛中，寄生虫在感染早期抑制了这种防御反应。
The outcome of infection in the host snail Biomphalaria glabrata with the digenean parasite Schistosoma mansoni is determined by the initial molecular interplay occurring between them. The mechanisms by which schistosomes evade snail immune recognition to ensure survival are not fully understood, but one possibility is that the snail internal defence system is manipulated by the schistosome enabling the parasite to establish infection. This study provides novel insights into the nature of schistosome resistance and susceptibility in B. glabrata at the transcriptomic level by simultaneously comparing gene expression in haemocytes from parasite-exposed and control groups of both schistosome-resistant and schistosome-susceptible strains, 2 h post exposure to S. mansoni miracidia, using an novel 5K cDNA microarray. Differences in gene expression, including those for immune/stress response, signal transduction and matrix/adhesion genes were identified between the two snail strains and tests for asymmetric distributions of gene function also identified immune-related gene expression in resistant snails, but not in susceptible. Gene set enrichment analysis revealed that genes involved in mitochondrial electron transport, ubiquinone biosynthesis and electron carrier activity were consistently up-regulated in resistant snails but down-regulated in susceptible. This supports the hypothesis that schistosome-resistant snails recognize schistosomes and mount an appropriate defence response, while in schistosome-susceptible snails the parasite suppresses this defence response, early in infection.
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影响因子：
2.1
作者：
BOSWELL, CA;BAYNE, CJ
通讯作者：
BAYNE, CJ
DOI：
10.1590/s0074-02762006000900027
发表时间：
2006-10-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Adema, Coen M;Luo, Mei-Zhong;Loker, Eric S
通讯作者：
Loker, Eric S
影响因子：
4
作者：
Hanington PC;Lun CM;Adema CM;Loker ES
通讯作者：
Loker ES
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
4
作者：
Bouchut, A;Roger, E;Mitta, G
通讯作者：
Mitta, G