Identification of novel bovine group A rotavirus G15P[14] strain from epizootic diarrhea of adult cows by de novo sequencing using a next-generation sequencer.
Identification of novel bovine group A rotavirus G15P[14] strain from epizootic diarrhea of adult cows by de novo sequencing using a next-generation sequencer.
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DOI：
10.1016/j.vetmic.2014.03.009
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发表时间：
2014-06-25
影响因子：
3.3
通讯作者：
Mizutani T
中科院分区：
文献类型：
--
作者：
Masuda T;Nagai M;Yamasato H;Tsuchiaka S;Okazaki S;Katayama Y;Oba M;Nishiura N;Sassa Y;Omatsu T;Furuya T;Koyama S;Shirai J;Taniguchi K;Fujii Y;Todaka R;Katayama K;Mizutani T
关于A组轮状病毒引起成年牛腹泻的报道很少。在这里，我们报告了一种新的牛A组轮状病毒的鉴定成年奶牛腹泻。2013年，在日本的一次成年奶牛腹泻流行病爆发中检测到A组轮状病毒，导致产奶量下降。使用下一代测序仪通过病毒宏基因组学对粪便样本进行的全面基因组分析显示，其具有未报告的基因型组合G15 P [14]。该菌株的基因组星座，即RVA/Cow-wt/JPN/Totori-SG/2013/G15 P [14]是G15-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3，分别代表VP 7-VP 4-VP 6-VP 1-VP 2-VP 3-NSP 1-NSP 2-NSP 3-NSP 4-NSP 5。鸟取-SG的每个基因片段与日本牛A组轮状病毒最密切相关，这表明鸟取-SG可能来自日本牛中流行的A组轮状病毒株的多重重配事件。常规诊断和宏基因组学检测均未检测到其他成年牛腹泻病原。使用新一代测序仪的病毒宏基因组学可用于表征粪便样品中的A组轮状病毒，并提供无偏见的病原体全面调查。
There are few reports describing diarrhea of adult cattle caused by group A rotaviruses. Here, we report the identification of a novel bovine group A rotavirus from diarrhea of adult cows. A group A rotavirus was detected from an epizootic outbreak of diarrhea in adult cows with a decrease in milk production in Japan in 2013. The comprehensive genomic analyses from fecal samples by viral metagenomics using a next-generation sequencer revealed that it had an unreported genotype combination G15P[14]. The genome constellation of this strain, namely, RVA/Cow-wt/JPN/Tottori-SG/2013/G15P[14] was G15-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3 representing VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5, respectively. Each gene segment of Tottori-SG was most closely related to Japanese bovine group A rotaviruses suggesting that Tottori-SG might have derived from multiple reassortment events from group A rotavirus strains circulating among Japanese cattle. No other diarrhea pathogen of adult cattle was detected by routine diagnosis and metagenomics. Viral metagenomics, using a next-generation sequencer, is useful to characterize group A rotaviruses from fecal samples and offers unbiased comprehensive investigations of pathogen.
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影响因子：
3.8
作者：
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通讯作者：
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