Needle electrode-based electromechanical reshaping of cartilage.
Needle electrode-based electromechanical reshaping of cartilage.
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DOI：
10.1007/s10439-010-0088-1
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发表时间：
2010-11
影响因子：
3.8
通讯作者：
Wong, Brian J. F.
中科院分区：
文献类型：
--
作者：
Manuel, Cyrus T.;Foulad, Allen;Protsenko, Dmitriy E.;Sepehr, Ali;Wong, Brian J. F.
关键词：
软骨的机电重塑(EMR)提供了一种替代传统外科技术来改变面部软骨形状的方法。EMR的最初实施要求表面电极与正在重塑的整个软骨区域直接接触。本研究评估了针状电极系统用于面软骨EMR的可行性，并评估了电极配置、电压和施加时间对形状变化的影响。用夹具将扁平兔鼻中隔软骨标本变形成90°弯曲，同时在插入软骨的针电极上施加恒定电压。系统地改变电极配置、电压(0-7.5V)和施加时间(1-900min)，以产生最有效的形状变化。在电压施加期间测量电流和温度，并根据保留的弯曲角评估得到的样品形状。为了验证EMR在临床上的可行性，我们使用了最有效和最实用的间隔软骨实验环境，对完整的兔耳和猪耳进行了体外重塑。用共聚焦显微镜结合活/死实验检测EMR后软骨细胞的活性。总体而言，随着电压的增加和施加时间的增加，软骨重塑增加。对于所测试的所有电极配置和施加时间，在6℃以下的发热量可以忽略不计(<；1°C)。在6℃时，使用最有效的电极配置时，弯曲角在施加时间2℃后开始显著增加，并在5℃以上开始趋于稳定。随着施加时间为2分钟的电压的变化，在5伏及以上时发生了显著的重塑，弯曲角度在6至7.5伏之间没有显著增加。结论：使用针电极可以有效地进行软骨移植和完整耳朵的机电重塑，温度升高可以忽略不计，细胞损伤在空间上是有限的。
Electromechanical reshaping (EMR) of cartilage provides an alternative to the classic surgical techniques of modifying the shape of facial cartilages. The original embodiment of EMR required surface electrodes to be in direct contact with the entire cartilage region being reshaped. This study evaluates the feasibility of using needle electrode systems for EMR of facial cartilage and evaluates the relationships between electrode configuration, voltage, and application time in effecting shape change. Flat rabbit nasal septal cartilage specimens were deformed by a jig into a 90° bend, while a constant electric voltage was applied to needle electrodes that were inserted into the cartilage. The electrode configuration, voltage (0–7.5 V), and application time (1–9 min) were varied systematically to create the most effective shape change. Electric current and temperature were measured during voltage application, and the resulting specimen shape was assessed in terms of retained bend angle. In order to demonstrate the clinical feasibility of EMR, the most effective and practical settings from the septal cartilage experimentation were used to reshape intact rabbit and pig ears ex vivo. Cell viability of the cartilage after EMR was determined using confocal microscopy in conjunction with a live/dead assay. Overall, cartilage reshaping increased with increased voltage and increased application time. For all electrode configurations and application times tested, heat generation was negligible (<1 °C) up to 6 V. At 6 V, with the most effective electrode configuration, the bend angle began to significantly increase after 2 min of application time and began to plateau above 5 min. As a function of voltage at 2 min of application time, significant reshaping occurred at and above 5 V, with no significant increase in the bend angle between 6 and 7.5 V. In conclusion, electromechanical reshaping of cartilage grafts and intact ears can be effectively performed with negligible temperature elevation and spatially limited cell injury using needle electrodes.
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影响因子：
2.4
作者：
Chae, Yongseok;Protsenko, Dmitriy;Holden, Paul K.;Chlebicki, Cara;Wong, Brian J. F.
通讯作者：
Wong, Brian J. F.
影响因子：
2.1
作者：
Jones, N;Sviridov, A;Lowe, J
通讯作者：
Lowe, J
影响因子：
2.4
作者：
Li, Chao;Protsenko, Dmitry E.;Wong, Brian
通讯作者：
Wong, Brian
DOI：
10.2500/105065801781543790
发表时间：
2001-03-01
期刊：
AMERICAN JOURNAL OF RHINOLOGY
影响因子：
--
作者：
Wong, BJF;Chao, KKH;Nelson, JS
通讯作者：
Nelson, JS
影响因子：
5.6
作者：
Stuck, BA;Sauter, A;Maurer, JT
通讯作者：
Maurer, JT