Comparative study of comprehensive gas chromatography-nitrogen chemiluminescence detection and gas chromatography-ion trap-tandem mass spectrometry for determining nicotine and carcinogen organic nitrogen compounds in thirdhand tobacco smoke.
Comparative study of comprehensive gas chromatography-nitrogen chemiluminescence detection and gas chromatography-ion trap-tandem mass spectrometry for determining nicotine and carcinogen organic nitrogen compounds in thirdhand tobacco smoke.
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气相色谱-氮化学发光综合检测法与气相色谱-离子阱-串联质谱法测定三手烟草烟气中尼古丁和致癌有机氮化合物的对比研究
DOI：
10.1016/j.chroma.2015.11.035
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Ramírez N
中科院分区：
文献类型：
--
作者：
Ramírez N
三手烟草烟雾（THS）构成了一个鲜为人知的途径，非吸烟者，特别是幼儿，烟草相关的致癌物质的暴露。然而，到目前为止，烟草烟雾中存在的大多数致癌物质尚未在THS中检测到，因此，THS健康风险的重要性仍然未知。在这项研究中，我们比较了两种分析方法的性能-一种是基于气相色谱-离子阱质谱检测（GC-IT-MS），另一种是基于全面的二维气相色谱-氮化学发光检测器（GC × GC-NCD）-用于同时测定，在沉降的房屋灰尘中，烟草烟雾中已经检测到的16种有机氮致癌物的存在。目标化合物包括4种芳香胺、2种硝基化合物、8种N-亚硝胺和2种烟草特有的亚硝胺，以及作为烟草标记物的尼古丁。以硅胶为净化吸附剂，乙酸乙酯为有机溶剂，采用密闭净化加压液相萃取法对粉尘样品进行提取，平均回收率为89%。虽然采用甲醇化学电离和串联质谱的GC-IT-MS表现良好，但优化的GC × GC-NCD检测限较低（4至22 ng g − 1），在低浓度水平下具有更好的重复性和再现性（%RSD <8%），因此，适用于测定这些不同类别的致癌物，而无需在GC分析之前进行衍生化。采用优化的PLE/GC × GC-NCD方法对吸烟者和非吸烟者家庭灰尘样品中的目标化合物进行定量分析。在吸烟者和非吸烟者的室内灰尘中检测到的致癌化合物中位数分别为3.8 μ g g − 1和1.1 μ g g − 1。在这项研究中，我们首次在沉降的房屋灰尘中检测到高度致癌的芳香胺和硝基化合物，补充了THS成分的知识状况，并提供了有关THS健康风险的新证据。
Thirdhand tobacco smoke (THS) constitutes a poorly understood pathway of exposure of non-smokers, especially toddlers, to tobacco-related carcinogens. However, to date most of the carcinogens present in tobacco smoke have not been detected in THS and, therefore, the significance of THS health risk is still unknown. In this study, we have compared the performance of two analytical methods – one based on gas chromatography coupled to ion trap mass spectrometry detection (GC-IT-MS) and the other on comprehensive two-dimensional gas chromatography coupled to a nitrogen chemiluminescence detector (GC × GC-NCD) – for simultaneously determining, in settled house dust, the presence of 16 organic nitrogen carcinogens already detected in tobacco smoke. The target compounds included four aromatic amines, two nitrocompounds, eight N-nitrosamines and two tobacco-specific nitrosamines, as well as nicotine as a tobacco marker. Dust samples were extracted using in-cell clean up pressurized liquid extraction with silica as clean up sorbent and ethyl acetate as the organic solvent, with average recovery of 89%. Although GC-IT-MS, using chemical ionization with methanol and tandem MS, performed well, the optimized GC × GC-NCD gave lower limits of detection (from 4 to 22 ng g−1) and better repeatability and reproducibility a low concentration levels (%RSD < 8%) and, therefore, was applicable for determining these different groups of carcinogens without the need for derivatization prior to the GC analysis.The performance of the optimized PLE/GC × GC-NCD method was tested by quantifying the target compounds in house dust samples from smokers’ and non-smokers’ homes. The median carcinogen compounds detected was 3.8 μg g−1and 1.1 μg g−1in smokers’ and non-smokers’ house dust, respectively. In this study, we have detected highly carcinogenic aromatic amines and nitro compounds for the first time in settled house dust complementing the state of knowledge of THS composition and providing fresh evidence about THS health risks.
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影响因子：
4.1
作者：
Ramirez, Noelia;Oezel, Mustafa Z.;Hamilton, Jacqueline F.
通讯作者：
Hamilton, Jacqueline F.
影响因子：
4.7
作者：
Quintana, Penelope J. E.;Matt, Georg E.;Hoh, Eunha
通讯作者：
Hoh, Eunha
影响因子：
3.1
作者：
Da Wu;Yifeng Lu;huaqing lin;Wanhong Zhou;Wenbo Gu
通讯作者：
Da Wu;Yifeng Lu;huaqing lin;Wanhong Zhou;Wenbo Gu
影响因子：
11.8
作者：
Ramirez, Noelia;Oezel, Mustafa Z.;Hamilton, Jacqueline F.
通讯作者：
Hamilton, Jacqueline F.
影响因子：
11.4
作者：
Hoh, Eunha;Hunt, Richard N.;Matt, Georg E.
通讯作者：
Matt, Georg E.