DRhoGEF2 regulates cellular tension and cell pulsations in the Amnioserosa during Drosophila dorsal closure.
DRhoGEF2 regulates cellular tension and cell pulsations in the Amnioserosa during Drosophila dorsal closure.
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DOI：
10.1371/journal.pone.0023964
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Jacinto A
中科院分区：
文献类型：
--
作者：
Azevedo D;Antunes M;Prag S;Ma X;Hacker U;Brodland GW;Hutson MS;Solon J;Jacinto A
在胚胎发育过程中，上皮细胞顶端缢痕的协调是一个基本的过程。在这里，我们表明，DRhoGEF 2是一个关键的调节器的顶端脉动和收缩的果蝇背关闭的神经鞘细胞。DRhoGEF 2突变的羊膜浆膜细胞表现出一致的浆膜搏动减少，而DRhoGEF 2在该组织中的过表达导致搏动收缩时间增加。我们探测了浆膜的物理性质，以表明DRhoGEF2突变细胞的平均张力低于野生型，并且DRhoGEF2的过表达导致组织比野生型更像固体。我们还观察到，在DRhoGEF2过表达细胞中，顶端肌动球蛋白聚结显著增加，这有助于产生更多的收缩力，导致具有比野生型更小的顶端表面的平滑肌细胞。相反，在DRhoGEF2突变体中，顶端肌动球蛋白聚结受损。这些结果确定DRhoGEF 2作为肌动球蛋白收缩机制的上游调节因子，该机制驱动浆膜细胞脉动和顶端收缩。
Coordination of apical constriction in epithelial sheets is a fundamental process during embryogenesis. Here, we show that DRhoGEF2 is a key regulator of apical pulsation and constriction of amnioserosal cells during Drosophila dorsal closure. Amnioserosal cells mutant for DRhoGEF2 exhibit a consistent decrease in amnioserosa pulsations whereas overexpression of DRhoGEF2 in this tissue leads to an increase in the contraction time of pulsations. We probed the physical properties of the amnioserosa to show that the average tension in DRhoGEF2 mutant cells is lower than wild-type and that overexpression of DRhoGEF2 results in a tissue that is more solid-like than wild-type. We also observe that in the DRhoGEF2 overexpressing cells there is a dramatic increase of apical actomyosin coalescence that can contribute to the generation of more contractile forces, leading to amnioserosal cells with smaller apical surface than wild-type. Conversely, in DRhoGEF2 mutants, the apical actomyosin coalescence is impaired. These results identify DRhoGEF2 as an upstream regulator of the actomyosin contractile machinery that drives amnioserosa cells pulsations and apical constriction.
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影响因子：
2
作者：
Ma, Xiaoyan;Lynch, Holley E.;Hutson, M. Shane
通讯作者：
Hutson, M. Shane
影响因子：
64.8
作者：
Martin, Adam C.;Kaschube, Matthias;Wieschaus, Eric F.
通讯作者：
Wieschaus, Eric F.
影响因子：
4.6
作者：
Homem, Catarina C. F.;Peifer, Mark
通讯作者：
Peifer, Mark
DOI：
10.1083/jcb.149.2.471
发表时间：
2000-04-17
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kiehart DP;Galbraith CG;Edwards KA;Rickoll WL;Montague RA
通讯作者：
Montague RA
影响因子：
10.5
作者：
Häcker, U;Perrimon, N
通讯作者：
Perrimon, N