RNA 5-Methylcytosine Modification Regulates Vegetative Development Associated with H3K27 Trimethylation in Arabidopsis.
RNA 5-Methylcytosine Modification Regulates Vegetative Development Associated with H3K27 Trimethylation in Arabidopsis.
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RNA 5-甲基胞嘧啶修饰调节拟南芥中与 H3K27 三甲基化相关的营养发育
DOI：
10.1002/advs.202204885
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发表时间：
2022-11-16
期刊：
影响因子：
15.1
通讯作者：
Pu, Li
中科院分区：
文献类型：
--
作者：
Zhang, Daolei;Guo, Weijun;Wang, Ting;Wang, Yifan;Le, Liang;Xu, Fan;Wu, Yue;Wuriyanghan, Hada;Sung, Zinmay Renee;Pu, Li
关键词：
RNA转录后甲基化是真核生物基因调控的重要机制。RNA甲基化和组蛋白修饰之间的串扰对于哺乳动物的染色质状态和基因表达至关重要。然而，它在植物中的机制还没有得到很好的理解。在这里，作者报告了拟南芥中RNA 5-胞嘧啶甲基化（m5 C）和组蛋白3赖氨酸27三甲基化（H3 K27 me 3）之间的全基因组相关性。植物特异性Polycomb组（PcG）蛋白胚胎花1（EMF 1）作为激活剂或抑制剂发挥双重作用。全转录组RNA m5 C分析显示，m5 C峰主要富集在野生型和emf 1突变体中缺乏H3 K27 me 3的染色质区域。EMF 1通过H3 K4 me 3抑制m5 C甲基转移酶tRNA特异性甲基转移酶4 B（TRM 4 B）的表达，不依赖于PcG介导的H3 K27 me 3机制。在emf 1突变体中，靶标上的5-胞嘧啶甲基化增加，从而减少光合作用和叶绿体基因的mRNA转录。此外，削弱EMF 1活性降低了PcG靶点（如淀粉基因）的H3 K27 me 3水平，这些基因在EMF 1突变体中被去抑制。通过m5 C和H3 K27 me 3的EMF 1介导的基因活性的促进和抑制都是正常营养生长所必需的。总的来说，这项研究揭示了一种以前未描述的RNA m5 C修饰和组蛋白修饰调节真核生物基因表达的表观遗传机制。植物特异性蛋白胚胎花1（EMF 1）介导RNA 5-胞嘧啶甲基化（m5 C）和组蛋白3赖氨酸27三甲基化（H3 K27 me 3）之间的全基因组相关性。EMF 1通过EMF 1介导的m5 C或H3 K27 me 3模块在调节靶基因表达中发挥双重作用，作为激活剂或抑制剂。动态表观基因组的这两个方面的整合是植物旺盛的营养生长所必需的。
Methylating RNA post‐transcriptionally is emerging as a significant mechanism of gene regulation in eukaryotes. The crosstalk between RNA methylation and histone modification is critical for chromatin state and gene expression in mammals. However, it is not well understood mechanistically in plants. Here, the authors report a genome‐wide correlation between RNA 5‐cytosine methylation (m5C) and histone 3 lysine27 trimethylation (H3K27me3) in Arabidopsis. The plant‐specific Polycomb group (PcG) protein EMBRYONIC FLOWER1 (EMF1) plays dual roles as activators or repressors. Transcriptome‐wide RNA m5C profiling revealed that m5C peaks are mostly enriched in chromatin regions that lacked H3K27me3 in both wild type and emf1 mutants. EMF1 repressed the expression of m5C methyltransferase tRNA specific methyltransferase 4B (TRM4B) through H3K4me3, independent of PcG‐mediated H3K27me3 mechanism. The 5‐Cytosine methylation on targets is increased in emf1 mutants, thereby decreased the mRNA transcripts of photosynthesis and chloroplast genes. In addition, impairing EMF1 activity reduced H3K27me3 levels of PcG targets, such as starch genes, which are de‐repressed in emf1 mutants. Both EMF1‐mediated promotion and repression of gene activities via m5C and H3K27me3 are required for normal vegetative growth. Collectively, t study reveals a previously undescribed epigenetic mechanism of RNA m5C modifications and histone modifications to regulate gene expression in eukaryotes. The plant‐specific protein EMBRYONIC FLOWER1 (EMF1) mediates a genome‐wide correlation between RNA 5‐cytosine methylation (m5C) and histone 3 lysine27 trimethylation (H3K27me3). EMF1 plays a dual role in regulating target gene expression as activator or repressor through EMF1‐mediated m5C or H3K27me3 modules. The integration of these two aspects of the dynamic epigenome is required for vigorous vegetative growth in plants.
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DOI：
10.1038/nrm4043
发表时间：
2015-09
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Du J;Johnson LM;Jacobsen SE;Patel DJ
通讯作者：
Patel DJ
影响因子：
10.5
作者：
Baile F;Gómez-Zambrano Á;Calonje M
通讯作者：
Calonje M
影响因子：
27.5
作者：
Cui, Xuean;Liang, Zhe;Yu, Hao
通讯作者：
Yu, Hao
影响因子：
7.2
作者：
Barlow, Denise P.;Bartolomei, Marisa S.
通讯作者：
Bartolomei, Marisa S.
DOI：
10.1199/tab.0128
发表时间：
2010-01-01
期刊：
The arabidopsis book
影响因子：
--
作者：
Alvarez-Venegas, Raul
通讯作者：
Alvarez-Venegas, Raul