Spatially Mapping the Baseline and Bisphenol-A Exposed Daphnia magna Lipidome Using Desorption Electrospray Ionization-Mass Spectrometry.
Spatially Mapping the Baseline and Bisphenol-A Exposed Daphnia magna Lipidome Using Desorption Electrospray Ionization-Mass Spectrometry.
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使用解吸电气电离 - 质谱法映射基线和双酚-A暴露的水达氏菌脂肪组。
DOI：
10.3390/metabo12010033
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发表时间：
2022-01-01
期刊：
影响因子：
4.1
通讯作者：
Viant MR
中科院分区：
文献类型：
--
作者：
Smith MJ;Weber RJM;Viant MR
非靶向脂质组学此前已被应用于对纤毛虫的研究和发现指示毒性的生物标记物。通常，液相色谱-质谱法被用来测量达夫类动物全身匀浆中脂质丰度的变化，每个匀浆的长度只有大约3毫米，这限制了对特定部位毒性的任何生化解释。在这里，我们应用大型水蚤的质谱学成像，将非靶向脂质组学与空间分辨率相结合，将分子扰动映射到定义的解剖区域。优化了解吸电喷雾质谱(DESI-MS)方法，并将其应用于暴露于双酚A(BPA)的甲虫组织切片中，与未暴露的对照进行比较，在每个切片中的每个像素(35微米/像素)处产生非靶向的质谱图。首先，利用主成分分析从不同的组织类型中识别出全身达夫类动物独特的脂类分布，特别是区分附肢、卵、眼睛和肠道。其次，绘制了正常卵子发育的四个阶段中脂体的变化图，然后研究了双酚A暴露对卵体脂体的影响。对脂质体的主要扰动被注释为三酰甘油酯和磷脂酰胆碱，这些类别中单个脂质物种的分布以离子图像的形式显示在大D全身切片上。使用优化的DESI-MS工作流程，我们生成了第一张大头金龟组织切片的离子图像，绘制了它们的基线和双酚A干扰的脂体。
Untargeted lipidomics has previously been applied to the study of daphnids and the discovery of biomarkers that are indicative of toxicity. Typically, liquid chromatography—mass spectrometry is used to measure the changes in lipid abundance in whole-body homogenates of daphnids, each only ca. 3 mm in length which limits any biochemical interpretation of site-specific toxicity. Here, we applied mass spectrometry imaging of Daphnia magna to combine untargeted lipidomics with spatial resolution to map the molecular perturbations to defined anatomical regions. A desorption electrospray ionization—mass spectrometry (DESI-MS) method was optimized and applied to tissue sections of daphnids exposed to bisphenol-A (BPA) compared to unexposed controls, generating an untargeted mass spectrum at each pixel (35 µm2/pixel) within each section. First, unique lipid profiles from distinct tissue types were identified in whole-body daphnids using principal component analysis, specifically distinguishing appendages, eggs, eye, and gut. Second, changes in the lipidome were mapped over four stages of normal egg development and then the effect of BPA exposure on the egg lipidome was characterized. The primary perturbations to the lipidome were annotated as triacylglycerides and phosphatidylcholine, and the distributions of the individual lipid species within these classes were visualized in whole-body D. magna sections as ion images. Using an optimized DESI-MS workflow, the first ion images of D. magna tissue sections were generated, mapping both their baseline and BPA-perturbed lipidomes.
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DOI：
10.1007/s13361-018-1979-x
发表时间：
2018-08
影响因子：
3.2
作者：
Bruinen AL;Fisher GL;Balez R;van der Sar AM;Ooi L;Heeren RMA
通讯作者：
Heeren RMA
影响因子：
8.9
作者：
Fuertes, Inmaculada;Jordao, Rita;Barata, Carlos
通讯作者：
Barata, Carlos
使用大气压基质辅助激光解吸电离质谱法对暴露于氟虫腈的斑马鱼进行磷脂成像
影响因子：
6.1
作者：
Liu, Wenjie;Nie, Hongxiang;Liu, Huwei
通讯作者：
Liu, Huwei
影响因子：
48
作者：
Palmer, Andrew;Phapale, Prasad;Alexandrov, Theodore
通讯作者：
Alexandrov, Theodore
影响因子：
8.8
作者：
Fuertes, Inmaculada;Barata, Carlos
通讯作者：
Barata, Carlos