Genetic diversity and epidemiology of human rhinovirus among children with severe acute respiratory tract infection in Guangzhou, China.
Genetic diversity and epidemiology of human rhinovirus among children with severe acute respiratory tract infection in Guangzhou, China.
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广州市重症急性呼吸道感染儿童人鼻病毒遗传多样性及流行病学
DOI：
10.1186/s12985-021-01645-6
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发表时间：
2021-08-23
期刊：
影响因子：
4.8
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Li W;Yu B;Zhou J;Wang Y;Xue B;Pan J;Ran Y;Yang X;Wang X;Yang F;Li H
研究背景人鼻病毒（Human rhinovirus，HRV）是婴幼儿急性呼吸道疾病的主要病毒之一。本研究旨在了解广州地区HRV的流行病学和系统发育特征。方法收集2018年8月至2019年12月期间因急性呼吸道感染住院的< 14岁儿童，分析其临床特征。结果655例标本中检出率为6.41%。HRV感染以2岁以下儿童多见（57.13%）。HRV-A和HRV-C在18例（45%）和22例（55%）标本中检出。40株HRV可分为29种基因型。HRV-C的分子进化速率为3.34 × 10- 3个替换/位点/年，比HRV-A的分子进化速率（7.79 × 10- 4个替换/位点/年）快。在HRV-C感染患者中，发生腹泻的儿童比HRV-A感染患者更常见。HRV-C检测组病毒载量高于HRV-A检测组（p= 0.0148）。中位峰值症状评分较高的患者与HRV-C感染相比，HRV-A（p= 0.0543），即使差异并不显着。结论本研究揭示了分子流行病学特征的HRV在中国南方呼吸道感染患者。HRV-C感染儿童的疾病特征较HRV-A严重，这可能与HRV-C感染患者的病毒载量较高有关。这些结果将为HRV感染的致病机制和治疗提供有价值的信息。
BackgroundHuman rhinovirus (HRV) is one of the major viruses of acute respiratory tract disease among infants and young children. This work aimed to understand the epidemiological and phylogenetic features of HRV in Guangzhou, China. In addition, the clinical characteristics of hospitalized children infected with different subtype of HRV was investigated.MethodsHospitalized children aged < 14 years old with acute respiratory tract infections were enrolled from August 2018 to December 2019. HRV was screened for by a real-time reverse-transcription PCR targeting the viral 5′UTR.ResultsHRV was detected in 6.41% of the 655 specimens. HRV infection was frequently observed in children under 2 years old (57.13%). HRV-A and HRV-C were detected in 18 (45%) and 22 (55%) specimens. All 40 HRV strains detected were classified into 29 genotypes. The molecular evolutionary rate of HRV-C was estimated to be 3.34 × 10–3substitutions/site/year and was faster than HRV-A (7.79 × 10–4substitutions/site/year). Children who experienced rhinorrhoea were more common in the HRV-C infection patients than HRV-A. The viral load was higher in HRV-C detection group than HRV-A detection group (p= 0.0148). The median peak symptom score was higher in patients with HRV-C infection as compared to HRV-A (p= 0.0543), even though the difference did not significance.ConclusionThis study revealed the molecular epidemiological characteristics of HRV in patients with respiratory infections in southern China. Children infected with HRV-C caused more severe disease characteristics than HRV-A, which might be connected with higher viral load in patients infected with HRV-C. These findings will provide valuable information for the pathogenic mechanism and treatment of HRV infection.
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DOI：
10.1183/09031936.00092410
发表时间：
2011-05
期刊：
The European respiratory journal
影响因子：
--
作者：
Bizzintino J;Lee WM;Laing IA;Vang F;Pappas T;Zhang G;Martin AC;Khoo SK;Cox DW;Geelhoed GC;McMinn PC;Goldblatt J;Gern JE;Le Souëf PN
通讯作者：
Le Souëf PN
影响因子：
14.2
作者：
Linder, Jodell E.;Kraft, David C.;Mohamed, Yassir;Lu, Zengqi;Heil, Luke;Tollefson, Sharon;Saville, Benjamin R.;Wright, Peter F.;Williams, John V.;Miller, E. Kathryn
通讯作者：
Miller, E. Kathryn
影响因子：
11.8
作者：
Calvo C;Garcia ML;Pozo F;Reyes N;Pérez-Breña P;Casas I
通讯作者：
Casas I
影响因子：
4.4
作者：
Gern, JE;Martin, MS;Lemanske, RF
通讯作者：
Lemanske, RF
影响因子：
11.8
作者：
Khetsuriani N;Lu X;Teague WG;Kazerouni N;Anderson LJ;Erdman DD
通讯作者：
Erdman DD