Silver nanoparticle loaded collagen/chitosan scaffolds promote wound healing via regulating fibroblast migration and macrophage activation
Silver nanoparticle loaded collagen/chitosan scaffolds promote wound healing via regulating fibroblast migration and macrophage activation
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负载银纳米颗粒的胶原/壳聚糖支架通过调节成纤维细胞迁移和巨噬细胞活化促进伤口愈合
DOI：
10.1038/s41598-017-10481-0
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发表时间：
2017-09
影响因子：
4.6
通讯作者：
Han Chunmao
中科院分区：
文献类型：
--
作者：
You Chuangang;Li Qiong;Wang Xingang;Wu Pan;Ho Jon Kee;Jin Ronghua;Zhang Liping;Shao Huawei;Han Chunmao
全层皮肤缺损的治疗带来了重大的临床挑战，包括感染和严重疤痕的风险。纳米银颗粒（NAg）作为一种有效的抗菌剂，为烧伤创面的治疗提供了一种新的途径。然而，详细的机制仍然未知。为此，我们构建了纳米银-胶原/壳聚糖杂化支架（NAg-CCS），并对其进行了体外划痕实验、免疫荧光染色和抗菌活性研究，同时将其应用于SD大鼠全层皮肤缺损的修复，观察其对创面愈合的影响。结果表明，10 ppm的NAg可促进成纤维细胞的迁移，并增加α-平滑肌肌动蛋白（α-SMA）的表达。此外，体内研究显示促炎和瘢痕相关因子以及α-SMA水平升高，而巨噬细胞活化标志物上调。超薄皮片移植后第60天，NAg-CCS再生皮肤具有与正常皮肤相似的结构。总之，我们证明了NAg-CCS具有杀菌、抗炎作用，并通过调节成纤维细胞迁移和巨噬细胞活化促进伤口愈合，使其成为伤口再生的理想真皮替代物。
Treatment of full-thickness skin defects poses significant clinical challenges including risk of infection and severe scaring. Silver nanoparticle (NAg), an effective antimicrobial agent, has provided a promising therapeutic method for burn wounds. However, the detailed mechanism remains unknown. Hence, we constructed a metallic nanosilver particles-collagen/chitosan hybrid scaffold (NAg-CCS) and investigated its potential effects on wound healing.In vitroscratch assay, immunofluorescence staining and antibacterial activity of the scaffold were all studied.In vivoNAg-CCS was applied in full-thickness skin defects in Sprague-Dawley (SD) rats and the therapeutic effects of treatment were evaluated. The results showed that NAg at a concentration of 10 ppm accelerated the migration of fibroblasts with an increase in expression of α-smooth muscle actin (α-SMA). Furthermore,in vivostudies showed increased levels of pro-inflammatory and scar-related factors as well as α-SMA, while markers for macrophage activation were up-regulated. On day 60 post transplantation of ultra-thin skin graft, the regenerated skin by NAg-CCS had a similar structure to normal skin. In summary, we demonstrated that NAg-CCS was bactericidal, anti-inflammatory and promoted wound healing potentially by regulating fibroblast migration and macrophage activation, making it an ideal dermal substitute for wound regeneration.
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纳米银粒子在大鼠体内的分布、易位和积累
影响因子：
--
作者：
Tang, Jinglong;Xiong, Ling;Xi, Tingfei
通讯作者：
Xi, Tingfei
DOI：
10.1073/pnas.0803343106
发表时间：
2009-01-06
影响因子：
11.1
作者：
Seno, Hiroshi;Miyoshi, Hiroyuki;Stappenbeck, Thaddeus S.
通讯作者：
Stappenbeck, Thaddeus S.
DOI：
10.1016/j.nano.2008.04.006
发表时间：
2008-09-01
影响因子：
5.4
作者：
Nadworny, Patricia L.;Wang, JianFei;Burrell, Robert E.
通讯作者：
Burrell, Robert E.
影响因子：
4.9
作者：
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通讯作者：
Brunette, Donald M.
影响因子：
2.9
作者：
Wiegand, Cornelia;Heinze, Thomas;Hipler, Uta-Christina
通讯作者：
Hipler, Uta-Christina