Expression of the human c-fms proto-oncogene in hematopoietic cells and its deletion in the 5q− syndrome
Expression of the human c-fms proto-oncogene in hematopoietic cells and its deletion in the 5q− syndrome
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人类c-fms原癌基因在造血细胞中的表达及其在5q−综合征中的缺失
DOI：
10.1016/0092-8674(85)90099-6
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发表时间：
1985
期刊：
影响因子：
64.5
通讯作者：
C. Sherr
中科院分区：
文献类型：
--
作者：
A. Nienhuis;H. Bunn;P. Turner;T. V. Gopal;W. Nash;S. O’Brien;C. Sherr
c-fms原癌基因在人骨髓和分化的血液单核细胞中表达，表明其基因产物在造血成熟中起作用。在HL-60细胞中未检测到c-fms mRNA，HL-60细胞是一种已建立的早幼粒细胞系，而c-fms表达出现在诱导后46小时，此时大多数细胞已分化为巨噬细胞。骨髓细胞中5号染色体（5 q-）的获得性缺失与血细胞生成异常有关。通过构建骨髓细胞与啮齿类动物细胞间的细胞杂种，分离出正常的5号染色体和Sq染色体。使用的选择性系统需要保留位于人5号染色体上的二氢叶酸还原酶的结构基因。对单个杂交克隆的DNA分析表明，5 q-缺失已经去除了c-fms基因。我们推测c-fms基因座的半合子导致造血成熟异常。
The c-fms proto-oncogene was shown to be expressed in human bone marrow and in differentiated blood mononuclear cells, suggesting that its gene product plays a role in hematopoietic maturation. The c-fms mRNA was not detected in HL-60 cells, an established promyelocytic line, whereas c-fms expression appeared 46 hr after induction when most cells had differentiated into macrophages. An acquired deletion of chromosome 5 (5q-) in bone marrow cells is associated with abnormalities in blood cell production. The normal 5 and Sq-chromosomes were segregated by construction of cell hybrids between bone marrow and rodent cells. A selective system was used that requires retention of the structural gene for dihydrofolate reductase, located on human chromosome 5. Analysis of DNA from individual hybrid clones revealed that the 5q-deletion had removed the c-fms gene. We postulate that hemizygosity at the c-fms locus leads to abnormalities in hematopoietic maturation.
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影响因子：
56.9
作者：
CANTRELL, DA;SMITH, KA
通讯作者：
SMITH, KA
影响因子：
56.9
作者：
DOOLITTLE, RF;HUNKAPILLER, MW;ANTONIADES, HN
通讯作者：
ANTONIADES, HN
影响因子：
56.9
作者：
BRODEUR, GM;SEEGER, RC;BISHOP, JM
通讯作者：
BISHOP, JM
影响因子：
56.9
作者：
C. W. Rettenmier;J. H. Chen;M. Roussel;C. Sherr
通讯作者：
C. W. Rettenmier;J. H. Chen;M. Roussel;C. Sherr
影响因子：
5.6
作者：
TUSHINSKI, RJ;STANLEY, ER
通讯作者：
STANLEY, ER