Receptor deorphanization in an echinoderm reveals kisspeptin evolution and relationship with SALMFamide neuropeptides.
Receptor deorphanization in an echinoderm reveals kisspeptin evolution and relationship with SALMFamide neuropeptides.
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DOI：
10.1186/s12915-022-01387-z
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发表时间：
2022-08-24
期刊：
影响因子：
5.4
通讯作者：
Elphick, Maurice R.
中科院分区：
文献类型：
--
作者：
Castelan, Nayeli Escudero;Semmens, Dean C.;Guerra, Luis Alfonso Yanez;Zandawala, Meet;Dos Reis, Mario;Slade, Susan E.;Scrivens, James H.;Zampronio, Cleidiane G.;Jones, Alexandra M.;Mirabeau, Olivier;Elphick, Maurice R.
Kispeptins是一种神经肽，通过G蛋白偶联受体介导的下丘脑促性腺激素释放激素的分泌来调节哺乳动物的生殖成熟。Kispeptin受体的系统发育分析表明，这种神经肽信号系统起源于Bilateria的一个共同祖先，但对无脊椎动物的Kispeptin信号系统知之甚少。与哺乳动物中单一的Kispeptin受体不同，本文报道了在后口无脊椎动物--海星Asterias Rubens(棘皮虫门)中发现了一个由11个Kispeptin类型受体组成的扩展家族。此外，从四种前体蛋白中提取的神经肽被确定为其中六种受体的配体。来源于两种前体蛋白(ArKPP1、ArKPP2)的一个或多个Kisspeptin样神经肽作为四个A.rubens Kisspeptin类型受体(ArKPR1、3、8、9)的配体。此外，棘皮动物中起肌肉松弛作用的神经肽家族(SALMFamide)是两个A.rubens kispeptin类型受体(ArKPR6，7)的配体。SALMFamide神经肽S1(或ArS1.4)和由S1前体蛋白衍生的七个神经肽(ArS1.1-ArS1.7)组成的“鸡尾酒”作为ArKPR7的配体。SALMFamide神经肽S2(或ArS2.3)和由S2前体蛋白衍生的8个神经肽(ArS2.1-ArS2.8)组成的“鸡尾酒”作为ArKPR6的配体。我们的发现揭示了海星中作为kisspeptin类型受体配体的神经肽的显着多样性，并为kisspeptin信号的进化提供了重要的新见解。此外，在哺乳动物中发现Kispeptins之前在海星中发现的神经肽SALMFamide与Kispeptins之间迄今未知的关系的发现，标志着Kispeptin信号研究的视角发生了根本性的变化。网上版载有补充材料，可在10.1186/s12915-022-01387-z查阅。
Kisspeptins are neuropeptides that regulate reproductive maturation in mammals via G-protein-coupled receptor-mediated stimulation of gonadotropin-releasing hormone secretion from the hypothalamus. Phylogenetic analysis of kisspeptin-type receptors indicates that this neuropeptide signaling system originated in a common ancestor of the Bilateria, but little is known about kisspeptin signaling in invertebrates. Contrasting with the occurrence of a single kisspeptin receptor in mammalian species, here, we report the discovery of an expanded family of eleven kisspeptin-type receptors in a deuterostome invertebrate — the starfish Asterias rubens (phylum Echinodermata). Furthermore, neuropeptides derived from four precursor proteins were identified as ligands for six of these receptors. One or more kisspeptin-like neuropeptides derived from two precursor proteins (ArKPP1, ArKPP2) act as ligands for four A. rubens kisspeptin-type receptors (ArKPR1,3,8,9). Furthermore, a family of neuropeptides that act as muscle relaxants in echinoderms (SALMFamides) are ligands for two A. rubens kisspeptin-type receptors (ArKPR6,7). The SALMFamide neuropeptide S1 (or ArS1.4) and a ‘cocktail’ of the seven neuropeptides derived from the S1 precursor protein (ArS1.1-ArS1.7) act as ligands for ArKPR7. The SALMFamide neuropeptide S2 (or ArS2.3) and a ‘cocktail’ of the eight neuropeptides derived from the S2 precursor protein (ArS2.1-ArS2.8) act as ligands for ArKPR6. Our findings reveal a remarkable diversity of neuropeptides that act as ligands for kisspeptin-type receptors in starfish and provide important new insights into the evolution of kisspeptin signaling. Furthermore, the discovery of the hitherto unknown relationship of kisspeptins with SALMFamides, neuropeptides that were discovered in starfish prior to the identification of kisspeptins in mammals, presents a radical change in perspective for research on kisspeptin signaling. The online version contains supplementary material available at 10.1186/s12915-022-01387-z.
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影响因子：
5.2
作者：
Elphick MR;Mirabeau O
通讯作者：
Mirabeau O
DOI：
10.1073/pnas.1834399100
发表时间：
2003-09-16
影响因子：
11.1
作者：
de Roux, N;Genin, E;Milgrom, E
通讯作者：
Milgrom, E
影响因子：
1.9
作者：
Cardoso, Joao C. R.;Felix, Rute C.;Power, Deborah M.
通讯作者：
Power, Deborah M.
影响因子：
10.7
作者：
Hoang DT;Chernomor O;von Haeseler A;Minh BQ;Vinh LS
通讯作者：
Vinh LS
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T